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Appl. Sci. 2018, 8(4), 560; https://doi.org/10.3390/app8040560

Development of ELISA-Like Fluorescence Assay for Melamine Detection Based on Magnetic Dummy Molecularly Imprinted Polymers

1,2,†
,
2,†
,
2
,
2,* and 1,*
1
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Vegetables Quality and Safety Control, Ministry of Agriculture of China, Ministry of Agriculture, Beijing 100081, China
2
Institute of Quality Standard and Testing Technology for Agro Products, Chinese Academy of Agricultural Sciences, Key Laboratory of Agrifood Safety and Quality, Ministry of Agriculture of China, Beijing 100081, China
Co-authors with equal contributions.
*
Authors to whom correspondence should be addressed.
Received: 5 February 2018 / Revised: 27 March 2018 / Accepted: 3 April 2018 / Published: 4 April 2018
(This article belongs to the Section Chemistry)
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Abstract

We present a directly competitive fluorescence assay for highly sensitive detection of melamine in milk using magnetic dummy molecularly imprinted polymers (MDMIPs). The detection principle is based on competitive binding between the fluorescent label and melamine on the MDMIPs. The fluorescent label was obtained by combining fluorescein isothiocyanate (FITC) with melamine in ethanol and water. MDMIPs were prepared on the surface of Fe3O4@SiO2 nanoparticles using 2,4-diamino-6-methyl-1,3,5-triazine as dummy template. The MDMIPs were characterized and their adsorption capacity was evaluated based on their static adsorption and Scatchard analysis. Results suggest that MDMIPs were successfully coated on the Fe3O4@SiO2 surface and had a core–shell structure. Adsorption experiments suggested that the MDMIPs had higher specific recognition capacities for melamine and FITC–melamine (FITC-Mel) than did magnetic dummy molecularly non-imprinted polymers. Competitive binding between FITC-Mel and melamine was performed under the optimum conditions to determine melamine quantitatively. The linear range of this fluorescence assay was 0.1–20 mg/L for melamine detection. The detection limit was 0.05 mg/L in negative milk samples. The assay was also successfully employed to detect melamine in spiked milk samples, with satisfactory recoveries, i.e., between 70.2% and 92.7%. View Full-Text
Keywords: ELISA-like fluorescence assay; magnetic dummy molecularly imprinted polymers; 2,4-diamino-6-methyl-1,3,5-triazine; fluorescein isothiocyanate; melamine ELISA-like fluorescence assay; magnetic dummy molecularly imprinted polymers; 2,4-diamino-6-methyl-1,3,5-triazine; fluorescein isothiocyanate; melamine
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Liu, G.; She, Y.; Hong, S.; Wang, J.; Xu, D. Development of ELISA-Like Fluorescence Assay for Melamine Detection Based on Magnetic Dummy Molecularly Imprinted Polymers. Appl. Sci. 2018, 8, 560.

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