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Appl. Sci. 2017, 7(8), 756; doi:10.3390/app7080756

Optimization of a Culture Medium Using the Taguchi Approach for the Production of Microorganisms Active in Odorous Compound Removal

1
Bionanopark, Laboratory of Industrial Biotechnology Ltd. Dubois 114/116, 93-465 Łódź, Poland
2
Institute of Materials Science and Engineering, Lodz University of Technology, Stefanowskiego 1/15, 90-924 Łódź, Poland
3
Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Wólczańska 171/173, 90-924 Łódź, Poland
4
JHJ Sp. z o.o., 63-308 Gizałki, Poland
Author has passed away.
*
Author to whom correspondence should be addressed.
Received: 21 June 2017 / Revised: 21 July 2017 / Accepted: 21 July 2017 / Published: 25 July 2017
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Abstract

The aim of this work was to develop the composition of a medium for the cultivation of six microbial strains forming a deodorizing consortium: Pseudomonas fluorescens, Enterococcus faecium, Bacillus subtilis, Bacillus megaterium, Leuconostoc mesenteroides and Lactobacillus plantarum. The study focused on the optimization of a highly efficient culture medium composed of readily available components of plant origin to maximize microbial biomass yields, and to create a less expensive alternative to the commercial Tryptic Soy Broth medium (TSB). After preliminary efficiency screening of all tested media components, we selected four substrates for further optimization—soy protein concentrate (SPC), glucose or sucrose, and phosphate salts. The final concentrations of all components were fine-tuned using the Taguchi design for experiments according to an L9 array. Taguchi optimization led to formulation of a culture medium, which was approximately 5 times cheaper than TSB (depending on the components used). Consequently, microbial biomass yields were improved by up to 15-fold (1564%), depending on the strain. The results obtained in the laboratory experiments were then confirmed in pilot- (42 L) and industrial- (300 L) scale fermentation. Our results show that this method of using a parallel culture microbioreactor with the Taguchi approach can be recommended for optimization of culture media based on substrates of plant origin. View Full-Text
Keywords: medium optimization; Taguchi method; bacterial biomass production; Biolector; components of plant origin medium optimization; Taguchi method; bacterial biomass production; Biolector; components of plant origin
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Makowski, K.; Matusiak, K.; Borowski, S.; Bielnicki, J.; Tarazewicz, A.; Maroszyńska, M.; Leszczewicz, M.; Powałowski, S.; Gutarowska, B. Optimization of a Culture Medium Using the Taguchi Approach for the Production of Microorganisms Active in Odorous Compound Removal. Appl. Sci. 2017, 7, 756.

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