Next Article in Journal
Structural Behavior of a Long-Span Partially Earth-Anchored Cable-Stayed Bridge during Installation of a Key Segment by Thermal Prestressing
Previous Article in Journal
A New Maximum Power Point Estimator Control Strategy to Maximize Output Power of the Double Stator Permanent Magnet Generator
Article Menu

Export Article

Open AccessArticle
Appl. Sci. 2016, 6(8), 232; doi:10.3390/app6080232

Laccase Immobilization on Poly(p-Phenylenediamine)/Fe3O4 Nanocomposite for Reactive Blue 19 Dye Removal

School of Basic Medical Sciences, Xinxiang Medical University, Jinsui Avenue 601, Xinxiang 453003, China
*
Author to whom correspondence should be addressed.
Academic Editor: Raed Abu-Reziq
Received: 5 July 2016 / Revised: 11 August 2016 / Accepted: 15 August 2016 / Published: 17 August 2016
(This article belongs to the Section Nanotechnology and Applied Nanosciences)
View Full-Text   |   Download PDF [3638 KB, uploaded 17 August 2016]   |  

Abstract

Magnetic poly(p-phenylenediamine) (PpPD) nanocomposite was synthesized via mixing p-phenylenediamine solution and Fe3O4 nanoparticles and used as a carrier for immobilized enzymes. Successful synthesis of PpPD/Fe3O4 nanofiber was confirmed by transmission electron microscopy and Fourier transform infrared spectroscopy. Laccase (Lac) was immobilized on the surface of PpPD/Fe3O4 nanofiber through covalent bonding for reactive blue 19 dye removal. The immobilized Lac-nanofiber conjugates could be recovered from the reaction solution using a magnet. The optimum reaction pH and temperature for the immobilized Lac were 3.5 and 65 °C, respectively. The storage, operational stability, and thermal stability of the immobilized Lac were higher than those of its free counterpart. The dye removal efficiency of immobilized Lac was about 80% in the first 1 h of incubation, while that of free Lac was about 20%. It was found that the unique electronic properties of PpPD might underlie the high dye removal efficiency of immobilized Lac. Over a period of repeated operation, the dye removal efficiency was above 90% during the first two cycles and remained at about 43% after eight cycles. Immobilized Lac on PpPD/Fe3O4 nanofiber showed high stability, easy recovery, reuse capabilities, and a high removal efficiency for reactive blue 19 dye; therefore, it provides an optional tool for dye removal from wastewater. View Full-Text
Keywords: laccase; poly(p-phenylenediamine); reactive blue 19; magnetic nanoparticles; removal; dye; wastewater treatment laccase; poly(p-phenylenediamine); reactive blue 19; magnetic nanoparticles; removal; dye; wastewater treatment
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Liu, Y.; Yan, M.; Geng, Y.; Huang, J. Laccase Immobilization on Poly(p-Phenylenediamine)/Fe3O4 Nanocomposite for Reactive Blue 19 Dye Removal. Appl. Sci. 2016, 6, 232.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Appl. Sci. EISSN 2076-3417 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top