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Appl. Sci. 2016, 6(6), 161; doi:10.3390/app6060161

Isolation, Identification, and Biotransformation of Teadenol A from Solid State Fermentation of Pu-erh Tea and In Vitro Antioxidant Activity

1
Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou 310008, China
2
College of Longrun Pu-erh Tea, Yunnan Agricultural University, Kunming, Yunnan 650201, China
3
Yunnan Research Center on Good Agricultural Practice for Dominant Chinese Medicinal Materials, Yunnan Agricultural University, Kunming, Yunnan 650201, China
4
Department of Horticultural Science, Faculty of Agriculture, Kagoshima University, Kagoshima 890-0065, Japan
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Helmut Martin Hügel
Received: 18 December 2015 / Revised: 11 May 2016 / Accepted: 11 May 2016 / Published: 26 May 2016
View Full-Text   |   Download PDF [4977 KB, uploaded 26 May 2016]   |  

Abstract

Post-fermented Pu-erh tea (PFPT) has several health benefits, however, little is known about the bioactive compounds. In this study, a PFPT compound was isolated by column chromatography and identified as Teadenol A by spectroscopic data analyses, including mass spectrometry and 1D and 2D NMR spectroscopy. Teadenol A in tea leaves was biotransformed by Aspergillus niger and A. tamari at 28 °C for 14 d at concentrations ranging from 9.85 ± 1.17 to 12.93 ± 0.38 mg/g. Additionally, the compound was detected in 22 commercial PFPTs at concentrations ranging from 0.17 ± 0.1 to 8.15 ± 0.1 mg/g. Teadenol A promoted the secretion of adiponectin and inhibited the expression of protein tyrosine phosphatase-1B. Antioxidant assays (e.g., 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, total antioxidant capacity (T-AOC), hydrogen donating ability, and superoxide anion radical scavenging capacity) revealed that Teadenol A has antioxidant properties. Therefore, Teadenol A is an important bio-active component of PFPT. View Full-Text
Keywords: Pu-erh tea; Teadenol A; antioxidant activity; Aspergillus niger; solid state fermentation Pu-erh tea; Teadenol A; antioxidant activity; Aspergillus niger; solid state fermentation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Su, X.-Q.; Zhang, G.-J.; Ma, Y.; Chen, M.; Chen, S.-H.; Duan, S.-M.; Wan, J.-Q.; Hashimoto, F.; Lv, H.-P.; Li, J.-H.; Lin, Z.; Zhao, M. Isolation, Identification, and Biotransformation of Teadenol A from Solid State Fermentation of Pu-erh Tea and In Vitro Antioxidant Activity. Appl. Sci. 2016, 6, 161.

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