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Appl. Sci. 2016, 6(2), 40; doi:10.3390/app6020040

A Microfluidic Device for Hydrodynamic Trapping and Manipulation Platform of a Single Biological Cell

1
Department of Control and Mechatronic Engineering, Faculty of Electrical Engineering, Universiti Teknologi Malaysia, Skudai, Johor 81310, Malaysia
2
Department of Micro-Nano Systems Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Aichi, Japan
3
Department of Bioscience and Health Sciences, Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia (UTM), Skudai, Johor 81310, Malaysia
*
Author to whom correspondence should be addressed.
Academic Editor: Fan-Gang Tseng
Received: 17 November 2015 / Revised: 16 December 2015 / Accepted: 5 January 2016 / Published: 1 February 2016
View Full-Text   |   Download PDF [5523 KB, uploaded 1 February 2016]   |  

Abstract

To perform specific analysis for the single cell, individual cells have to be captured and separated from each other before further treatments and analysis can be carried out. This paper presents the design, simulation, fabrication, and testing of a microfluidic device for trapping a single cell/particle based on a hydrodynamic technique. A T-channel trapping chip has been proposed to provide single-cell trapping and consequently could be a platform for cell treatments and manipulations. A finite element T-channel trapping model was developed using Abaqus FEA™ software to observe it’s trapping ability by optimizing the channel’s geometry and RhMain/RhTrap ratio. A proof of concept demonstration for cell trapping in the T-channel model was presented in the simulation analysis and experimental work using HUVEC cell aggregate. The T-channel was found to be able to trap a single cell via the hydrodynamic trapping concept using an appropriate channel geometry and RhMain/RhTrap ratio. The proposed T-channel single-cell trapping has potential application for single cell characterization and single 3D cell aggregates treatments and analysis. View Full-Text
Keywords: single cell; 3D cell aggregate; hydrodynamic trapping single cell; 3D cell aggregate; hydrodynamic trapping
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MDPI and ACS Style

Ahmad Khalili, A.; Ahmad, M.R.; Takeuchi, M.; Nakajima, M.; Hasegawa, Y.; Mohamed Zulkifli, R. A Microfluidic Device for Hydrodynamic Trapping and Manipulation Platform of a Single Biological Cell. Appl. Sci. 2016, 6, 40.

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