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Appl. Sci. 2016, 6(12), 432;

A Label-Free Aptamer-Based Fluorescent Assay for Cadmium Detection

1,2,* , 1,2
Beijing Research Center for Agricultural Standards and Testing, Beijing 100097, China
Beijing Municipal Key Laboratory of Agriculture Environment Monitoring, Beijing 100097, China
Author to whom correspondence should be addressed.
Academic Editor: Samuel B. Adeloju
Received: 1 November 2016 / Revised: 2 December 2016 / Accepted: 10 December 2016 / Published: 14 December 2016
(This article belongs to the Section Chemistry)
View Full-Text   |   Download PDF [1295 KB, uploaded 14 December 2016]   |  


Selective detection of ultratrace amounts of cadmium (Cd2+) is extremely important for food safety and environmental monitoring because of its toxicity and widespread use. In this work, we developed a facile, rapid, sensitive, and highly selective method for the detection of Cd2+ based on a label-free aptasensor using an unmodified double-stranded deoxyribonucleic acid-specific dye (PicoGreen). The linear range was 0.10–100 µg/mL, and the detection limit (0.038 ng/mL) was lower than the guideline from the World Health Organization for Cd2+ in drinking water (3 ng/mL). The sensor exhibited excellent selectivity towards Cd2+ ions. We tested the aptasensor in application to a series of real water samples spiked with different concentrations of Cd2+. Compared with atomic absorption spectrometry, the results showed good tolerance to the matrix effect. The developed approach shows great potential for on-site and high-throughput analysis in routine monitoring. View Full-Text
Keywords: aptamer; biosensor; cadmium; PicoGreen; fluorescence aptamer; biosensor; cadmium; PicoGreen; fluorescence

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Luan, Y.; Lu, A.; Chen, J.; Fu, H.; Xu, L. A Label-Free Aptamer-Based Fluorescent Assay for Cadmium Detection. Appl. Sci. 2016, 6, 432.

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