A Label-Free Aptamer-Based Fluorescent Assay for Cadmium Detection
AbstractSelective detection of ultratrace amounts of cadmium (Cd2+) is extremely important for food safety and environmental monitoring because of its toxicity and widespread use. In this work, we developed a facile, rapid, sensitive, and highly selective method for the detection of Cd2+ based on a label-free aptasensor using an unmodified double-stranded deoxyribonucleic acid-specific dye (PicoGreen). The linear range was 0.10–100 µg/mL, and the detection limit (0.038 ng/mL) was lower than the guideline from the World Health Organization for Cd2+ in drinking water (3 ng/mL). The sensor exhibited excellent selectivity towards Cd2+ ions. We tested the aptasensor in application to a series of real water samples spiked with different concentrations of Cd2+. Compared with atomic absorption spectrometry, the results showed good tolerance to the matrix effect. The developed approach shows great potential for on-site and high-throughput analysis in routine monitoring. View Full-Text
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Luan, Y.; Lu, A.; Chen, J.; Fu, H.; Xu, L. A Label-Free Aptamer-Based Fluorescent Assay for Cadmium Detection. Appl. Sci. 2016, 6, 432.
Luan Y, Lu A, Chen J, Fu H, Xu L. A Label-Free Aptamer-Based Fluorescent Assay for Cadmium Detection. Applied Sciences. 2016; 6(12):432.Chicago/Turabian Style
Luan, Yunxia; Lu, Anxiang; Chen, Jiayi; Fu, Hailong; Xu, Li. 2016. "A Label-Free Aptamer-Based Fluorescent Assay for Cadmium Detection." Appl. Sci. 6, no. 12: 432.
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