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Appl. Sci. 2016, 6(12), 432; doi:10.3390/app6120432

A Label-Free Aptamer-Based Fluorescent Assay for Cadmium Detection

1,2
,
1,2,* , 1,2
,
1,2
and
1,2
1
Beijing Research Center for Agricultural Standards and Testing, Beijing 100097, China
2
Beijing Municipal Key Laboratory of Agriculture Environment Monitoring, Beijing 100097, China
*
Author to whom correspondence should be addressed.
Academic Editor: Samuel B. Adeloju
Received: 1 November 2016 / Revised: 2 December 2016 / Accepted: 10 December 2016 / Published: 14 December 2016
(This article belongs to the Section Chemistry)
View Full-Text   |   Download PDF [1295 KB, uploaded 14 December 2016]   |  

Abstract

Selective detection of ultratrace amounts of cadmium (Cd2+) is extremely important for food safety and environmental monitoring because of its toxicity and widespread use. In this work, we developed a facile, rapid, sensitive, and highly selective method for the detection of Cd2+ based on a label-free aptasensor using an unmodified double-stranded deoxyribonucleic acid-specific dye (PicoGreen). The linear range was 0.10–100 µg/mL, and the detection limit (0.038 ng/mL) was lower than the guideline from the World Health Organization for Cd2+ in drinking water (3 ng/mL). The sensor exhibited excellent selectivity towards Cd2+ ions. We tested the aptasensor in application to a series of real water samples spiked with different concentrations of Cd2+. Compared with atomic absorption spectrometry, the results showed good tolerance to the matrix effect. The developed approach shows great potential for on-site and high-throughput analysis in routine monitoring. View Full-Text
Keywords: aptamer; biosensor; cadmium; PicoGreen; fluorescence aptamer; biosensor; cadmium; PicoGreen; fluorescence
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MDPI and ACS Style

Luan, Y.; Lu, A.; Chen, J.; Fu, H.; Xu, L. A Label-Free Aptamer-Based Fluorescent Assay for Cadmium Detection. Appl. Sci. 2016, 6, 432.

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