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Appl. Sci. 2015, 5(4), 881-893; doi:10.3390/app5040881

Partial Purification and Characterization of a Thermostable β-Mannanase from Aspergillus foetidus

1
Laboratory of Enzymology, Cellular Biology Department, University of Brasília, Brasília 70910-900, Brazil
2
Federal Institute of Education, Science and Technology of Goiás, Rio Verde, Goiás 75901-970, Brazil
3
Department of Biology, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo 14040-901, Brazil
*
Author to whom correspondence should be addressed.
Academic Editors: Helmut Martin Hügel and Takayoshi Kobayashi
Received: 23 July 2015 / Revised: 14 October 2015 / Accepted: 15 October 2015 / Published: 20 October 2015
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Abstract

An extracellular β-mannanase was isolated from samples of crude extract of the mesophilic fungus Aspergillus foetidus grown on soybean husk as a carbon source. The induction profile showed that β-mannanase reached a maximum activity level (2.0 IU/mL) on the 15th day of cultivation. The enzyme was partially purified by ultrafiltration and gel filtration chromatography procedures and was named Man 58. Sodium dodecyl sulfate-polyacrilamide electrophoresis and zymogram analysis of Man 58 showed two bands of approximately 43 and 45 kDa with β-mannanase activity. Ultrafiltration showed that β-mannanase activity was only detected in the concentrated sample. Man 58 was most active at 60 °C and at pH 4.0. It was thermostable in the temperature range of 40–60 °C for eleven days, and the half-life at 70 °C was ten days. Man 58 showed Km and Vmax values of 3.29 mg/mL and 1.76 IU/mL respectively, with locust bean gum as a substrate. It was mostly activated by FeSO4 and CoCl2 and inhibited by MgSO4, FeCl3, CuSO4, MgCl2, ZnCl2, ZnSO4, CaCl2, CuCl2, KCl and ethylenediaminetetraacetic acid (EDTA). Phenolic compounds did not inhibit the enzyme. On the other hand, auto-hydrolysis liquor showed an inhibitory effect on Man 58 activity. View Full-Text
Keywords: soybean husk; β-mannanase; Aspergillus foetidus soybean husk; β-mannanase; Aspergillus foetidus
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

de Marco, J.C.I.; Neto, G.P.S.; Castro, C.F.S.; Michelin, M.; Polizeli, M.L.T.M.; Filho, E.X.F. Partial Purification and Characterization of a Thermostable β-Mannanase from Aspergillus foetidus. Appl. Sci. 2015, 5, 881-893.

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