Tissue Engineering 3D Porous Scaffolds Prepared from Electrospun Recombinant Human Collagen (RHC) Polypeptides/Chitosan Nanofibers
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Fabrication of the Chitosan/RHC Nanofibers
2.3. Fabrication of the 3D Nanofibrous Scaffolds
2.4. Characterization
2.4.1. Morphology Analysis of the Nanofibers and Scaffolds
2.4.2. Open Porosity
2.4.3. Swelling Ability
2.4.4. Degradation
2.4.5. Fourier Transform Infrared Spectroscopy (FT-IR)
2.4.6. Mechanical Properties
2.5. In Vitro Cell Studies
2.6. Gene Expression Analysis
2.7. Statistical Analysis
3. Results and Discussion
3.1. Structure of the Nanofibers and 3D Nanofibrous Scaffolds
3.2. Swelling Ability and Degradation
3.3. Fourier Transform Infrared Spectroscopy (FT-IR)
3.4. Mechanical Properties of the 3D Nanofibrous Scaffolds
3.5. Cell Proliferation and Morphology
3.6. Gene Expression Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Deng, A.; Yang, Y.; Du, S. Tissue Engineering 3D Porous Scaffolds Prepared from Electrospun Recombinant Human Collagen (RHC) Polypeptides/Chitosan Nanofibers. Appl. Sci. 2021, 11, 5096. https://doi.org/10.3390/app11115096
Deng A, Yang Y, Du S. Tissue Engineering 3D Porous Scaffolds Prepared from Electrospun Recombinant Human Collagen (RHC) Polypeptides/Chitosan Nanofibers. Applied Sciences. 2021; 11(11):5096. https://doi.org/10.3390/app11115096
Chicago/Turabian StyleDeng, Aipeng, Yang Yang, and Shimei Du. 2021. "Tissue Engineering 3D Porous Scaffolds Prepared from Electrospun Recombinant Human Collagen (RHC) Polypeptides/Chitosan Nanofibers" Applied Sciences 11, no. 11: 5096. https://doi.org/10.3390/app11115096