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Med. Sci. 2014, 2(2), 70-81; doi:10.3390/medsci2020070
Article

In Vitro Efficient Expansion of Tumor Cells Deriving from Different Types of Human Tumor Samples

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Received: 14 November 2013 / Revised: 3 March 2014 / Accepted: 4 March 2014 / Published: 26 March 2014
(This article belongs to the Special Issue Recent Advances in Cellular Immunotherapy)
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Abstract

Obtaining human tumor cell lines from fresh tumors is essential to advance our understanding of antitumor immune surveillance mechanisms and to develop new ex vivo strategies to generate an efficient anti-tumor response. The present study delineates a simple and rapid method for efficiently establishing primary cultures starting from tumor samples of different types, while maintaining the immuno-histochemical characteristics of the original tumor. We compared two different strategies to disaggregate tumor specimens. After short or long term in vitro expansion, cells analyzed for the presence of malignant cells demonstrated their neoplastic origin. Considering that tumor cells may be isolated in a closed system with high efficiency, we propose this methodology for the ex vivo expansion of tumor cells to be used to evaluate suitable new drugs or to generate tumor-specific cytotoxic T lymphocytes or vaccines.
Keywords: tumor cell line; in vitro model; cell culture; immunomagnetic selection tumor cell line; in vitro model; cell culture; immunomagnetic selection
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Turin, I.; Schiavo, R.; Maestri, M.; Luinetti, O.; Bello, B.D.; Paulli, M.; Dionigi, P.; Roccio, M.; Spinillo, A.; Ferulli, F.; Tanzi, M.; Maccario, R.; Montagna, D.; Pedrazzoli, P. In Vitro Efficient Expansion of Tumor Cells Deriving from Different Types of Human Tumor Samples. Med. Sci. 2014, 2, 70-81.

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