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Microorganisms 2017, 5(3), 60; doi:10.3390/microorganisms5030060

Proteomic Characterization of Armillaria mellea Reveals Oxidative Stress Response Mechanisms and Altered Secondary Metabolism Profiles

1
Department of Biology, Maynooth University, Maynooth, Co. Kildare W23 F2H6, Ireland
2
European Bioinformatics Institute, Hinxton, Cambridge CB10 1SD, UK
*
Author to whom correspondence should be addressed.
Received: 3 August 2017 / Revised: 8 September 2017 / Accepted: 13 September 2017 / Published: 17 September 2017
(This article belongs to the Special Issue Applications of Genomics to Infectious Disease of Plants)
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Abstract

Armillaria mellea is a major plant pathogen. Yet, the strategies the organism uses to infect susceptible species, degrade lignocellulose and other plant material and protect itself against plant defences and its own glycodegradative arsenal are largely unknown. Here, we use a combination of gel and MS-based proteomics to profile A. mellea under conditions of oxidative stress and changes in growth matrix. 2-DE and LC-MS/MS were used to investigate the response of A. mellea to H2O2 and menadione/FeCl3 exposure, respectively. Several proteins were detected with altered abundance in response to H2O2, but not menadione/FeCl3 (i.e., valosin-containing protein), indicating distinct responses to these different forms of oxidative stress. One protein, cobalamin-independent methionine synthase, demonstrated a common response in both conditions, which may be a marker for a more general stress response mechanism. Further changes to the A. mellea proteome were investigated using MS-based proteomics, which identified changes to putative secondary metabolism (SM) enzymes upon growth in agar compared to liquid cultures. Metabolomic analyses revealed distinct profiles, highlighting the effect of growth matrix on SM production. This establishes robust methods by which to utilize comparative proteomics to characterize this important phytopathogen. View Full-Text
Keywords: Armillaria mellea; basidiomycete proteomics; oxidative stress; fungal proteomics; methionine synthase; polyamines; secondary metabolism Armillaria mellea; basidiomycete proteomics; oxidative stress; fungal proteomics; methionine synthase; polyamines; secondary metabolism
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MDPI and ACS Style

Collins, C.; Hurley, R.; Almutlaqah, N.; O’Keeffe, G.; Keane, T.M.; Fitzpatrick, D.A.; Owens, R.A. Proteomic Characterization of Armillaria mellea Reveals Oxidative Stress Response Mechanisms and Altered Secondary Metabolism Profiles. Microorganisms 2017, 5, 60.

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