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Microorganisms 2017, 5(2), 16; doi:10.3390/microorganisms5020016

Insights on Klebsiella pneumoniae Biofilms Assembled on Different Surfaces Using Phenotypic and Genotypic Approaches

1
Instituto Nacional de Saúde Dr Ricardo Jorge, Departamento de Saúde Ambiental, Unidade de Investigação e Desenvolvimento-Lisboa, Avenida Padre Cruz, 1649-016 Lisboa, Portugal
2
Universidade de Lisboa, Instituto Superior Técnico, Departamento de Engenharia Química, Avenida Rovisco Pais, 1049-001 Lisboa, Portugal
3
Instituto Nacional de Saúde Dr Ricardo Jorge, Departamento de Doenças Infeciosas, Núcleo de Bioinformática, Avenida Padre Cruz, 1649-016 Lisboa, Portugal
4
Universidade de Lisboa, Faculdade de Farmácia, Av Prof Gama Pinto, 1649-003 Lisboa, Portugal
*
Author to whom correspondence should be addressed.
Academic Editor: Rikke Louise Meyer
Received: 8 February 2017 / Revised: 6 March 2017 / Accepted: 29 March 2017 / Published: 3 April 2017
(This article belongs to the Special Issue The Biofilm Matrix)
View Full-Text   |   Download PDF [6876 KB, uploaded 3 April 2017]   |  

Abstract

Klebsiella pneumoniae is a prominent etiological agent of healthcare associated infections (HAIs). In this context, multidrug-resistant and biofilm-producing bacteria are of special public health concern due to the difficulties associated with treatment of human infections and eradication from hospital environments. Here, in order to study the impact of medical devices-associated materials on the biofilm dynamics, we performed biofilm phenotypic analyses through a classic and a new scanning electron microscopy (SEM) technique for three multidrug-resistant K. pneumoniae isolates growing on polystyrene and silicone. We also applied whole-genome sequencing (WGS) to search for genetic clues underlying biofilm phenotypic differences. We found major differences in the extracellular polymeric substances (EPS) content among the three strains, which were further corroborated by in-depth EPS composition analysis. WGS analysis revealed a high nucleotide similarity within the core-genome, but relevant differences in the accessory genome that may account for the detected biofilm phenotypic dissimilarities, such as genes already associated with biofilm formation in other pathogenic bacteria (e.g., genes coding haemogglutinins and haemolysins). These data reinforce that the research efforts to defeat bacterial biofilms should take into account that their dynamics may be contingent on the medical devices-associated materials. View Full-Text
Keywords: biofilms; Klebsiella pneumoniae; electron microscopy; extracellular polymeric substances (EPS); whole genome sequencing (WGS); healthcare associated infections (HAIs) biofilms; Klebsiella pneumoniae; electron microscopy; extracellular polymeric substances (EPS); whole genome sequencing (WGS); healthcare associated infections (HAIs)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Bandeira, M.; Borges, V.; Gomes, J.P.; Duarte, A.; Jordao, L. Insights on Klebsiella pneumoniae Biofilms Assembled on Different Surfaces Using Phenotypic and Genotypic Approaches. Microorganisms 2017, 5, 16.

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