Identification and Characterization of Spontaneous Auxotrophic Mutants in Fusarium langsethiae
AbstractAnalysis of 49 strains of Fusarium langsethiae originating from northern Europe (Russia, Finland, Sweden, UK, Norway, and Latvia) revealed the presence of spontaneous auxotrophic mutants that reflect natural intraspecific diversity. Our investigations detected that 49.0% of F. langsethiae strains were auxotrophic mutants for biotin, and 8.2% of the strains required thiamine as a growth factor. They failed to grow on vitamin-free media. For both prototrophic and auxotrophic strains, no growth defect was observed in rich organic media. Without essential vitamins, a significant reduction in the growth of the auxotrophic strains results in a decrease of the formation of T-2 toxin and diacetoxyscirpenol. In addition, all analysed F. langsethiae strains were distinguished into two subgroups based on PCR product sizes. According to our results, 26 and 23 strains of F. langsethiae belong to subgroups I and II respectively. We determined that the deletion in the intergenic spacer (IGS) region of the rDNA of F. langsethiae belonging to subgroup II is linked with temperature sensitivity and causes a decrease in strain growth at 30 °C. Four thiamine auxotrophic strains were found in subgroup I, while 21 biotin auxotrophic strains were detected in subgroups II. To the best of our knowledge, the spontaneous mutations in F. langsethiae observed in the present work have not been previously reported. View Full-Text
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Gavrilova, O.; Skritnika, A.; Gagkaeva, T. Identification and Characterization of Spontaneous Auxotrophic Mutants in Fusarium langsethiae. Microorganisms 2017, 5, 14.
Gavrilova O, Skritnika A, Gagkaeva T. Identification and Characterization of Spontaneous Auxotrophic Mutants in Fusarium langsethiae. Microorganisms. 2017; 5(2):14.Chicago/Turabian Style
Gavrilova, Olga; Skritnika, Anna; Gagkaeva, Tatiana. 2017. "Identification and Characterization of Spontaneous Auxotrophic Mutants in Fusarium langsethiae." Microorganisms 5, no. 2: 14.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.