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Microorganisms 2016, 4(4), 38; doi:10.3390/microorganisms4040038

Flavin-Dependent Redox Transfers by the Two-Component Diketocamphane Monooxygenases of Camphor-Grown Pseudomonas putida NCIMB 10007

1
College of Life and Environmental Sciences, University of Exeter, Exeter EX4 4QG, UK
2
School of Chemistry, Cardiff University, Cardiff CF10 3AT, UK
Deceased, February 2008.
*
Author to whom correspondence should be addressed.
Academic Editor: Spiros N. Agathos
Received: 29 August 2016 / Revised: 30 September 2016 / Accepted: 3 October 2016 / Published: 13 October 2016
View Full-Text   |   Download PDF [1337 KB, uploaded 13 October 2016]   |  

Abstract

The progressive titres of key monooxygenases and their requisite native donors of reducing power were used to assess the relative contribution of various camphor plasmid (CAM plasmid)- and chromosome-coded activities to biodegradation of (rac)-camphor at successive stages throughout growth of Pseudomonas putida NCIMB 10007 on the bicylic monoterpenoid. A number of different flavin reductases (FRs) have the potential to supply reduced flavin mononucleotide to both 2,5- and 3,6-diketocamphane monooxygenase, the key isoenzymic two-component monooxygenases that delineate respectively the (+)- and (−)-camphor branches of the convergent degradation pathway. Two different constitutive chromosome-coded ferric reductases able to act as FRs can serve such as role throughout all stages of camphor-dependent growth, whereas Fred, a chromosome-coded inducible FR can only play a potentially significant role in the relatively late stages. Putidaredoxin reductase, an inducible CAM plasmid-coded flavoprotein that serves an established role as a redox intermediate for plasmid-coded cytochrome P450 monooxygenase also has the potential to serve as an important FR for both diketocamphane monooxygenases (DKCMOs) throughout most stages of camphor-dependent growth. View Full-Text
Keywords: diketocamphane monooxygenase; flavin reductase; putidaredoxin reductase; Pseudomonas putida NCIMB 10007 diketocamphane monooxygenase; flavin reductase; putidaredoxin reductase; Pseudomonas putida NCIMB 10007
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Willetts, A.; Kelly, D. Flavin-Dependent Redox Transfers by the Two-Component Diketocamphane Monooxygenases of Camphor-Grown Pseudomonas putida NCIMB 10007. Microorganisms 2016, 4, 38.

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