Fed-Batch Production of Bacterial Ghosts Using Dielectric Spectroscopy for Dynamic Process Control
AbstractThe Bacterial Ghost (BG) platform technology evolved from a microbiological expression system incorporating the ϕX174 lysis gene E. E-lysis generates empty but structurally intact cell envelopes (BGs) from Gram-negative bacteria which have been suggested as candidate vaccines, immunotherapeutic agents or drug delivery vehicles. E-lysis is a highly dynamic and complex biological process that puts exceptional demands towards process understanding and control. The development of a both economic and robust fed-batch production process for BGs required a toolset capable of dealing with rapidly changing concentrations of viable biomass during the E-lysis phase. This challenge was addressed using a transfer function combining dielectric spectroscopy and soft-sensor based biomass estimation for monitoring the rapid decline of viable biomass during the E-lysis phase. The transfer function was implemented to a feed-controller, which followed the permittivity signal closely and was capable of maintaining a constant specific substrate uptake rate during lysis phase. With the described toolset, we were able to increase the yield of BG production processes by a factor of 8–10 when compared to currently used batch procedures reaching lysis efficiencies >98%. This provides elevated potentials for commercial application of the Bacterial Ghost platform technology. View Full-Text
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Meitz, A.; Sagmeister, P.; Lubitz, W.; Herwig, C.; Langemann, T. Fed-Batch Production of Bacterial Ghosts Using Dielectric Spectroscopy for Dynamic Process Control. Microorganisms 2016, 4, 18.
Meitz A, Sagmeister P, Lubitz W, Herwig C, Langemann T. Fed-Batch Production of Bacterial Ghosts Using Dielectric Spectroscopy for Dynamic Process Control. Microorganisms. 2016; 4(2):18.Chicago/Turabian Style
Meitz, Andrea; Sagmeister, Patrick; Lubitz, Werner; Herwig, Christoph; Langemann, Timo. 2016. "Fed-Batch Production of Bacterial Ghosts Using Dielectric Spectroscopy for Dynamic Process Control." Microorganisms 4, no. 2: 18.
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