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Microorganisms 2016, 4(1), 17; doi:10.3390/microorganisms4010017

Process Recovery after CaO Addition Due to Granule Formation in a CSTR Co-Digester—A Tool to Influence the Composition of the Microbial Community and Stabilize the Process?

1
GFZ German Research Centre for Geosciences, Section 5.3 Geomicrobiology, Telegrafenberg, 14473 Potsdam, Germany
2
Institute of Geological Sciences, Jagiellonian University, 30-063 Krakow, Poland
3
Dresden Groundwater Research Center e. V., Meranerstr. 10, 01217 Dresden, Germany
4
Department of Engineering and Natural Sciences, University of Applied Sciences Merseburg, Eberhard-Leibnitz-Str. 2, 06217 Merseburg, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Willy Verstraete
Received: 23 November 2015 / Revised: 4 March 2016 / Accepted: 8 March 2016 / Published: 17 March 2016
(This article belongs to the Special Issue Microbial Resource Management)
View Full-Text   |   Download PDF [1357 KB, uploaded 17 March 2016]   |  

Abstract

The composition, structure and function of granules formed during process recovery with calcium oxide in a laboratory-scale fermenter fed with sewage sludge and rapeseed oil were studied. In the course of over-acidification and successful process recovery, only minor changes were observed in the bacterial community of the digestate, while granules appeared during recovery. Fluorescence microscopic analysis of the granules showed a close spatial relationship between calcium and oil and/or long chain fatty acids. This finding further substantiated the hypothesis that calcium precipitated with carbon of organic origin and reduced the negative effects of overloading with oil. Furthermore, the enrichment of phosphate minerals in the granules was shown, and molecular biological analyses detected polyphosphate-accumulating organisms as well as methanogenic archaea in the core. Organisms related to Methanoculleus receptaculi were detected in the inner zones of a granule, whereas they were present in the digestate only after process recovery. This finding indicated more favorable microhabitats inside the granules that supported process recovery. Thus, the granule formation triggered by calcium oxide addition served as a tool to influence the composition of the microbial community and to stabilize the process after overloading with oil. View Full-Text
Keywords: over-acidification; process recovery; phosphate accumulating organisms; granule formation over-acidification; process recovery; phosphate accumulating organisms; granule formation
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MDPI and ACS Style

Liebrich, M.; Kleyböcker, A.; Kasina, M.; Miethling-Graff, R.; Kassahun, A.; Würdemann, H. Process Recovery after CaO Addition Due to Granule Formation in a CSTR Co-Digester—A Tool to Influence the Composition of the Microbial Community and Stabilize the Process? Microorganisms 2016, 4, 17.

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