Toll-Like Receptor-4 Dependent Intestinal and Systemic Sequelae Following Peroral Campylobacter coli Infection of IL10 Deficient Mice Harboring a Human Gut Microbiota
Abstract
:1. Introduction
2. Results
2.1. Gastrointestinal Pathogen Loads Following Peroral C. coli Infection of Human Microbiota-Associated TLR4-Deficient IL10-/- Mice
2.2. Commensal Gut Microbiota Changes Following C. coli Infection of Human Microbiota-Associated TLR4-Deficient IL10-/- Mice
2.3. Kinetic Survey of Clinical Signs Exerted by C. coli-Infected Human Microbiota-Associated TLR4-Deficient IL10-/- Mice
2.4. Colonic Apoptotic and Immune Cell Responses Following C. coli Infection of Human Microbiota-Associated TLR4-Deficient IL10-/- Mice
2.5. Intestinal and Systemic Pro-Inflammatory Cytokine Secretion Following C. coli Infection of Human Microbiota-Associated TLR4-Deficient IL10-/- Mice
3. Discussion
4. Materials and Methods
4.1. Ethics Statement
4.2. Generation of Secondary Abiotic Mice
4.3. Reassociation of Secondary Abiotic Mice with a Human Gut Microbiota by Fecal Microbiota Transplantation
4.4. C. coli Infection and Gastrointestinal Colonization
4.5. Cultural and Culture-Independent (i.e., Molecular) Survey of the Human Donor Suspensions and Gut Microbiota
4.6. Clinical Conditions
4.7. Sampling Procedures
4.8. Immunohistochemistry
4.9. Pro-Inflammatory Cytokines in Intestinal and Serum Samples
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Kløve, S.; Genger, C.; Mousavi, S.; Weschka, D.; Bereswill, S.; Heimesaat, M.M. Toll-Like Receptor-4 Dependent Intestinal and Systemic Sequelae Following Peroral Campylobacter coli Infection of IL10 Deficient Mice Harboring a Human Gut Microbiota. Pathogens 2020, 9, 386. https://doi.org/10.3390/pathogens9050386
Kløve S, Genger C, Mousavi S, Weschka D, Bereswill S, Heimesaat MM. Toll-Like Receptor-4 Dependent Intestinal and Systemic Sequelae Following Peroral Campylobacter coli Infection of IL10 Deficient Mice Harboring a Human Gut Microbiota. Pathogens. 2020; 9(5):386. https://doi.org/10.3390/pathogens9050386
Chicago/Turabian StyleKløve, Sigri, Claudia Genger, Soraya Mousavi, Dennis Weschka, Stefan Bereswill, and Markus M. Heimesaat. 2020. "Toll-Like Receptor-4 Dependent Intestinal and Systemic Sequelae Following Peroral Campylobacter coli Infection of IL10 Deficient Mice Harboring a Human Gut Microbiota" Pathogens 9, no. 5: 386. https://doi.org/10.3390/pathogens9050386