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Pathogens 2016, 5(2), 39; doi:10.3390/pathogens5020039

A Cellular GWAS Approach to Define Human Variation in Cellular Pathways Important to Inflammation

1
Department of Microbiology, Department of Immunology, Department of Medicine, Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
2
Department of Microbiology, University of Washington, Seattle, WA 98195, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Lawrence S. Young
Received: 11 September 2015 / Revised: 7 April 2016 / Accepted: 21 April 2016 / Published: 26 April 2016
(This article belongs to the Special Issue Molecular Aspects of Urinary Tract Infection)
View Full-Text   |   Download PDF [637 KB, uploaded 26 April 2016]   |  

Abstract

An understanding of common human diversity in innate immune pathways should be beneficial in understanding autoimmune diseases, susceptibility to infection, and choices of anti-inflammatory treatment. Such understanding could also result in definition of currently unknown components of human inflammation pathways. A cellular genome-wide association studies (GWAS) platform, termed Hi-HOST (High-throughput human in vitro susceptibility testing), was developed to assay in vitro cellular phenotypes of infection in genotyped lymphoblastoid cells from genetically diverse human populations. Hi-HOST allows for measurement of multiple host and pathogen parameters of infection/inflammation including: bacterial invasion and intracellular replication, host cell death, and cytokine production. Hi-HOST has been used to successfully define a significant portion of the heritable human diversity in inflammatory cell death in response to Salmonella typhimurium. It also led to the discovery of genetic variants important to protection against systemic inflammatory response syndrome (SIRS) and protection against death and bacteremia in individuals with SIRS. Our laboratory is currently using this platform to define human diversity in autophagy and the NLPR3 inflammasome pathways, and to define new components that can impact the expression of phenotypes related to these pathways. View Full-Text
Keywords: GWAS; Hi-HOST; host; lymphoblastoid; pathogen; pyroptosis; SIRS GWAS; Hi-HOST; host; lymphoblastoid; pathogen; pyroptosis; SIRS
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Miller, S.I.; Chaudhary, A. A Cellular GWAS Approach to Define Human Variation in Cellular Pathways Important to Inflammation. Pathogens 2016, 5, 39.

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