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Pathogens 2015, 4(4), 752-763; doi:10.3390/pathogens4040752

Candida albicans Shed Msb2 and Host Mucins Affect the Candidacidal Activity of Salivary Hst 5

1
Department of Oral Biology, School of Dental Medicine, University at Buffalo, Buffalo, NY 14214, USA
2
Department of Pharmaceutical Sciences, University at Buffalo, Buffalo, NY 14214, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Sarah Gaffen
Received: 18 August 2015 / Revised: 26 October 2015 / Accepted: 28 October 2015 / Published: 30 October 2015
(This article belongs to the Special Issue Candida Albicans Infections)
View Full-Text   |   Download PDF [1367 KB, uploaded 30 October 2015]   |  

Abstract

Salivary Histatin 5 (Hst 5) is an antimicrobial peptide that exhibits potent antifungal activity towards Candida albicans, the causative agent of oral candidiasis. However, it exhibits limited activity in vivo, largely due to inactivation by salivary components of both host and pathogen origin. Proteins secreted by C. albicans during infection such as secreted aspartyl proteases (Saps) and shed mucin Msb2 can reduce Hst 5 activity; and human salivary mucins, while suggested to protect Hst 5 from proteolytic degradation, can entrap peptides into mucin gels, thereby reducing bioavailability. We show here that Sap6 that is secreted during hyphal growth reduces Hst 5 activity, most likely a result of proteolytic degradation of Hst 5 since this effect is abrogated with heat inactivated Sap 6. We further show that just like C. albicans shedding Msb2, mammalian mucins, fetuin and porcine gut mucin (that is related to salivary mucins), also reduce Hst 5 activity. However, we identify mucin-like protein-induced changes in C. albicans cell morphology and aggregation patterns, suggesting that the effect of such proteins on Hst 5 cannot be interpreted independently of their effect on yeast cells. View Full-Text
Keywords: Candida albicans; oral candidiasis; Histatin 5; saliva; mucins; secreted aspartyl proteases Candida albicans; oral candidiasis; Histatin 5; saliva; mucins; secreted aspartyl proteases
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Puri, S.; Friedman, J.; Saraswat, D.; Kumar, R.; Li, R.; Ruszaj, D.; Edgerton, M. Candida albicans Shed Msb2 and Host Mucins Affect the Candidacidal Activity of Salivary Hst 5. Pathogens 2015, 4, 752-763.

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