Detection of Virulence-Associated Genes among Brucella melitensis and Brucella abortus Clinical Isolates in Greece, 2001–2022
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Isolates
2.2. Identification
2.3. Molecular Detection of Virulence Genes
2.4. Reference Strains
2.5. Statistical Evaluation
3. Results and Discussion
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Sequence | Amplicon (bp) | Reference |
---|---|---|---|
omp19 | F: 5′-TGATGGGAATTTCAAAAGCA-3 R: 5′-GTTTCCGGGTCAGATCAGC-3′ | 550 | [3] |
wbkA | F: 5′-AATGACTTCCGCTGCCATAG-3′ R: 5′-ATGAGCGAGGACATGAGCTT-3′ | 931 | [3] |
manA | F: 5′-TCGATCCAGAAACCCAGTTC-3′ R: 5′-CATACACCACGATCCACTGC-3′ | 271 | [10] |
mviN | F: 5′-GCAGATCAACCTGCTCATCA-3′ R: 5′-GGCCATAGATCGCCAGAATA-3′ | 344 | [3] |
ure | F: 5′-GCTTGCCCTTGAATTCCTTTGTGG-3′ R: 5′-ATCTGCGAATTTGCCGGACTCTAT-3′ | 2100 | [11] |
perA | F: 5′-GGAACGGTGGCACTACATCT-3′ R: 5′-GGCTCTCTGTGTTCCGAGTT-3′ | 716 | [3] |
cbg | F: 5′- GAATTCGCCAATGAGGAAAA-3′ R: 5′- ACGATATCGGATGCGAAAAG-3′ | 575 | [10] |
virB | F: 5′- CGCTGATCTATAATTAAGGCTA -3′ R: 5′- TGCGACTGCCTCCTATCGTC-3′ | 881 | [10] |
B. melitensis (No) | omp19 | wbkA | manA | mviN | ure | perA | cbg | virB |
---|---|---|---|---|---|---|---|---|
305 | + | + | + | + | + | + | + | + |
7 | + | + | + | + | Negative | + | + | + |
2 | + | Negative | + | + | Negative | + | + | + |
1 | + | + | + | + | + | + | Negative | Negative |
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Papaparaskevas, J.; Procopiou, A.; Routsias, J.; Vrioni, G.; Tsakris, A. Detection of Virulence-Associated Genes among Brucella melitensis and Brucella abortus Clinical Isolates in Greece, 2001–2022. Pathogens 2023, 12, 1274. https://doi.org/10.3390/pathogens12111274
Papaparaskevas J, Procopiou A, Routsias J, Vrioni G, Tsakris A. Detection of Virulence-Associated Genes among Brucella melitensis and Brucella abortus Clinical Isolates in Greece, 2001–2022. Pathogens. 2023; 12(11):1274. https://doi.org/10.3390/pathogens12111274
Chicago/Turabian StylePapaparaskevas, Joseph, Alexandra Procopiou, John Routsias, Georgia Vrioni, and Athanasios Tsakris. 2023. "Detection of Virulence-Associated Genes among Brucella melitensis and Brucella abortus Clinical Isolates in Greece, 2001–2022" Pathogens 12, no. 11: 1274. https://doi.org/10.3390/pathogens12111274