Next Article in Journal / Special Issue
Epigenetic Regulation of a Heat-Activated Retrotransposon in Cruciferous Vegetables
Previous Article in Journal
Driver or Passenger: Epigenomes in Alzheimer’s Disease
Article Menu

Export Article

Open AccessArticle
Epigenomes 2017, 1(1), 6; doi:10.3390/epigenomes1010006

The Pea (Pisum sativum L.) Rogue Paramutation is Accompanied by Alterations in the Methylation Pattern of Specific Genomic Sequences

Laboratory of Genomics and Genetic Improvement, FCT, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal
*
Author to whom correspondence should be addressed.
Academic Editor: Etienne Bucher
Received: 28 February 2017 / Revised: 5 May 2017 / Accepted: 11 May 2017 / Published: 18 May 2017
(This article belongs to the Special Issue Plant Epigenome Dynamics)
View Full-Text   |   Download PDF [1668 KB, uploaded 18 May 2017]   |  

Abstract

The spontaneous emergence among common pea (Pisum sativum L.) cultivars of off-type rogue plants exhibiting leaves with narrower and pointed leaflets and stipules and the non-Mendelian inheritance of this new phenotype were first described in the early 20th century. However, so far, no studies at the molecular level of this first identified case of paramutation have been carried out. In this study, we show for the first time that the pea rogue paramutation is accompanied by alterations in the methylation status of specific genomic sequences. Although, no significant differences were observed in the genome-wide DNA methylation in leaves of non-rogue cv. Onward in comparison to its rogue paramutant line JI2723, 22 DNA sequences were identified by methylation-sensitive amplified fragment length polymorphisms (MS-AFLP) analysis as differentially methylated in the two epigenomes. Mitotically inherited through all leaf tissues, the differential methylation patterns were also found to be meiotically inherited and conserved in pollen grains for 12 out of the 22 sequences. Fourteen of the sequences were successfully amplified in cDNA but none of them exhibited significant differential expression in the two contrasting epigenotypes. The further exploitation of the present research results on the way towards the elucidation of the molecular mechanisms behind this interesting epigenetic phenomenon is discussed. View Full-Text
Keywords: paramutation; epigenetics; rogue plants; Pisum sativum; DNA methylation paramutation; epigenetics; rogue plants; Pisum sativum; DNA methylation
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Santo, T.E.; Pereira, R.J.; Leitão, J.M. The Pea (Pisum sativum L.) Rogue Paramutation is Accompanied by Alterations in the Methylation Pattern of Specific Genomic Sequences. Epigenomes 2017, 1, 6.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Epigenomes EISSN 2075-4655 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top