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Insects 2018, 9(3), 110; https://doi.org/10.3390/insects9030110

Nutritional Quality during Development Alters Insulin-Like Peptides’ Expression and Physiology of the Adult Yellow Fever Mosquito, Aedes aegypti

1
Department of Agriculture, Nutrition, and Veterinary Sciences, University of Nevada, Reno, NV 89557, USA
2
Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV 89557, USA
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 5 June 2018 / Revised: 30 July 2018 / Accepted: 22 August 2018 / Published: 30 August 2018
(This article belongs to the Special Issue Integrative Mosquito Biology: From Molecules to Ecosystems)
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Abstract

Mosquitoes have distinct developmental and adult life history, and the vectorial capacity of females has been shown to be affected by the larval nutritional environment. However, little is known about the effect of developmental nutrition on insulin-signaling and nutrient storage. In this study, we used Aedes aegypti, the yellow fever mosquito, to determine whether larval nutrition affects insulin gene expression. We also determined the traits regulated by insulin signaling, such as stored-nutrient levels and fecundity. We raised mosquito larvae on two different diets, containing either high protein or high carbohydrates. Development on a high-carbohydrate diet resulted in several life-history phenotypes indicative of suboptimal conditions, including increased developmental time and decreased fecundity. Additionally, our data showed that insulin transcript levels are affected by a high-carbohydrate diet during development. Females, not males, reared on high-carbohydrate diets had much higher transcript levels of insulin-like peptide 3 (ILP3), a mosquito equivalent of human insulin, and these females more readily converted sugar meals into lipids. We also found that AaILP4, not AaILP3, transcript levels were much higher in the males after a sugar meal, suggesting sex-specific differences in the insulin-signaling pathway. Our findings suggest a conserved mechanism of carbohydrate-mediated hyperinsulinemia in animals. View Full-Text
Keywords: insulin-signaling; insulin receptor; Aedes aegypti; mosquitoes; insulin; insulin-like peptides; nutrition; hyperinsulinemia; larval-diet; metabolic reserves insulin-signaling; insulin receptor; Aedes aegypti; mosquitoes; insulin; insulin-like peptides; nutrition; hyperinsulinemia; larval-diet; metabolic reserves
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Pooraiiouby, R.; Sharma, A.; Beard, J.; Reyes, J.; Nuss, A.; Gulia-Nuss, M. Nutritional Quality during Development Alters Insulin-Like Peptides’ Expression and Physiology of the Adult Yellow Fever Mosquito, Aedes aegypti. Insects 2018, 9, 110.

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