Implications of Possible HBV-Driven Regulation of Gene Expression in Stem Cell-like Subpopulation of Huh-7 Hepatocellular Carcinoma Cell Line
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Separation of Huh-7 CSCs
2.3. Plasmid Transfection
2.4. Invasion Assay
2.5. PCR Analyses for EMT Markers
2.6. miRNome Analysis
2.7. RNA-Seq Data Analysis
2.8. Phylogenetic Analysis of miRNAs
2.9. Genomic Database Analysis
2.10. Putative Protein Structure Homology Analysis
2.11. Cancer Cell Line Database Analysis
2.12. Statistics
3. Results
3.1. miRNA Profile upon Orai1 and STIM1 Overexpression
3.2. Phylogenetic Analysis of miRNAs
3.3. Genomic Database Analysis of Putative HBV Regions
3.4. Structural Homology Analysis
3.5. E-Cadherin Expression Changes
3.6. Expression Patterns of miR3653 Target Genes
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Demir, A.B.; Benvenuto, D.; Karacicek, B.; Erac, Y.; Spoto, S.; Angeletti, S.; Ciccozzi, M.; Tosun, M. Implications of Possible HBV-Driven Regulation of Gene Expression in Stem Cell-like Subpopulation of Huh-7 Hepatocellular Carcinoma Cell Line. J. Pers. Med. 2022, 12, 2065. https://doi.org/10.3390/jpm12122065
Demir AB, Benvenuto D, Karacicek B, Erac Y, Spoto S, Angeletti S, Ciccozzi M, Tosun M. Implications of Possible HBV-Driven Regulation of Gene Expression in Stem Cell-like Subpopulation of Huh-7 Hepatocellular Carcinoma Cell Line. Journal of Personalized Medicine. 2022; 12(12):2065. https://doi.org/10.3390/jpm12122065
Chicago/Turabian StyleDemir, Ayse Banu, Domenico Benvenuto, Bilge Karacicek, Yasemin Erac, Silvia Spoto, Silvia Angeletti, Massimo Ciccozzi, and Metiner Tosun. 2022. "Implications of Possible HBV-Driven Regulation of Gene Expression in Stem Cell-like Subpopulation of Huh-7 Hepatocellular Carcinoma Cell Line" Journal of Personalized Medicine 12, no. 12: 2065. https://doi.org/10.3390/jpm12122065