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Diagnostics 2015, 5(1), 10-26; doi:10.3390/diagnostics5010010

Mini-P-gp and P-gp Co-Expression in Brown Trout Erythrocytes: A Prospective Blood Biomarker of Aquatic Pollution

1
ERTICa—EA 4677, Département Génie Biologique, Institut Universitaire de Technologie, Clermont Université—Université d'Auvergne, Ensemble universitaire des Cézeaux, B.P. 86-63172 Aubiere, France
2
ERTICa—EA 4677, Centre Jean Perrin, Clermont Université—Université d'Auvergne, 58 rue Montalembert, B.P. 392-63011 Clermont-Ferrand, France
3
LMGE—UMR CNRS 6023, Clermont Université—Université Blaise Pascal, B.P. 80026-63171 Aubiere, France
4
Fédération pour la Pêche et la Protection du Milieu Aquatique du Puy de Dôme (F.P.P.M.A. 63), 14 Allée des Eaux et Forêts, Site de Marmilhat Sud, 63370 Lempdes, France
5
Rovaltain Research Company, 1 avenue de la gare, B.P. 10313, 26958 Valence Cedex 09, France
*
Author to whom correspondence should be addressed.
Academic Editor: Ludmilla A. Morozova-Roche
Received: 10 October 2014 / Accepted: 19 December 2014 / Published: 12 January 2015
(This article belongs to the Special Issue Biomarkers in Blood)
View Full-Text   |   Download PDF [1108 KB, uploaded 12 January 2015]   |  

Abstract

In aquatic organisms, such as fish, blood is continually exposed to aquatic contaminants. Multidrug Resistance (MDR) proteins are ubiquitous detoxification membrane pumps, which recognize various xenobiotics. Moreover, their expression is induced by a large class of drugs and pollutants. We have highlighted the co-expression of a mini P-gp of 75 kDa and a P-gp of 140 kDa in the primary culture of brown trout erythrocytes and in the erythrocytes of wild brown trout collected from three rivers in the Auvergne region of France. In vitro experiments showed that benzo[a]pyrene, a highly toxic pollutant model, induced the co-expression of mini-P-gp and P-gp in trout erythrocytes in a dose-dependent manner and relay type response. Similarly, in the erythrocytes of wild brown trout collected from rivers contaminated by a mixture of PAH and other multi-residues of pesticides, mini-P-gp and P-gp were able to modulate their expression, according to the nature of the pollutants. The differential and complementary responses of mini-P-gp and P-gp in trout erythrocytes suggest the existence in blood cells of a real protective network against xenobiotics/drugs. This property could be exploited to develop a blood biomarker of river pollution. View Full-Text
Keywords: blood biomarker; erythrocytes; brown trout; mini-P-gp; P-gp; river pollution blood biomarker; erythrocytes; brown trout; mini-P-gp; P-gp; river pollution
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Valton, E.; Amblard, C.; Desmolles, F.; Combourieu, B.; Penault-Llorca, F.; Bamdad, M. Mini-P-gp and P-gp Co-Expression in Brown Trout Erythrocytes: A Prospective Blood Biomarker of Aquatic Pollution. Diagnostics 2015, 5, 10-26.

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