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Special Issue: Microfluidic Lab-on-a-Chip Platforms for High-Performance Diagnostics
Diagnostics 2012, 2(4), 72-82; doi:10.3390/diagnostics2040072
Article

Gold Nanoparticles-Coated SU-8 for Sensitive Fluorescence-Based Detections of DNA

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Received: 26 September 2012; in revised form: 24 October 2012 / Accepted: 26 November 2012 / Published: 29 November 2012
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Abstract: SU-8 epoxy-based negative photoresist has been extensively employed as a structural material for fabrication of numerous biological microelectro-mechanical systems (Bio-MEMS) or lab-on-a-chip (LOC) devices. However, SU-8 has a high autofluorescence level that limits sensitivity of microdevices that use fluorescence as the predominant detection workhorse. Here, we show that deposition of a thin gold nanoparticles layer onto the SU-8 surface significantly reduces the autofluorescence of the coated SU-8 surface by as much as 81% compared to bare SU-8. Furthermore, DNA probes can easily be immobilized on the Au surface with high thermal stability. These improvements enabled sensitive DNA detection by simple DNA hybridization down to 1 nM (a two orders of magnitude improvement) or by solid-phase PCR with sub-picomolar sensitivity. The approach is simple and easy to perform, making it suitable for various Bio-MEMs and LOC devices that use SU-8 as a structural material.
Keywords: gold nanoparticles; SU-8; autofluorescence; solid-phase PCR amplification; DNA hybridization gold nanoparticles; SU-8; autofluorescence; solid-phase PCR amplification; DNA hybridization
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Cao, C.; Birtwell, S.W.; Høgberg, J.; Morgan, H.; Wolff, A.; Bang, D.D. Gold Nanoparticles-Coated SU-8 for Sensitive Fluorescence-Based Detections of DNA. Diagnostics 2012, 2, 72-82.

AMA Style

Cao C, Birtwell SW, Høgberg J, Morgan H, Wolff A, Bang DD. Gold Nanoparticles-Coated SU-8 for Sensitive Fluorescence-Based Detections of DNA. Diagnostics. 2012; 2(4):72-82.

Chicago/Turabian Style

Cao, Cuong; Birtwell, Sam W.; Høgberg, Jonas; Morgan, Hywel; Wolff, Anders; Bang, Dang D. 2012. "Gold Nanoparticles-Coated SU-8 for Sensitive Fluorescence-Based Detections of DNA." Diagnostics 2, no. 4: 72-82.


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