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Life 2016, 6(3), 35; doi:10.3390/life6030035

The Use of a Fractional Factorial Design to Determine the Factors That Impact 1,3-Propanediol Production from Glycerol by Halanaerobium hydrogeniformans

1
Department of Biological Sciences, Missouri University of Science & Technology, Rolla, MO 65401, USA
2
Department of Chemical and Biochemical Engineering, Missouri University of Science & Technology, Rolla, MO 65401, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Rafael Montalvo-Rodríguez
Received: 8 July 2016 / Revised: 11 August 2016 / Accepted: 15 August 2016 / Published: 20 August 2016
View Full-Text   |   Download PDF [403 KB, uploaded 20 August 2016]   |  

Abstract

In recent years, biodiesel, a substitute for fossil fuels, has led to the excessive production of crude glycerol. The resulting crude glycerol can possess a high concentration of salts and an alkaline pH. Moreover, current crude glycerol purification methods are expensive, rendering this former commodity a waste product. However, Halanaerobium hydrogeniformans, a haloalkaliphilic bacterium, possesses the metabolic capability to convert glycerol into 1,3-propanediol, a valuable commodity compound, without the need for salt dilution or adjusting pH when grown on this waste. Experiments were performed with different combinations of 24 medium components to determine their impact on the production of 1,3-propanediol by using a fractional factorial design. Tested medium components were selected based on data from the organism’s genome. Analysis of HPLC data revealed enhanced production of 1,3-propanediol with additional glycerol, pH, vitamin B12, ammonium ions, sodium sulfide, cysteine, iron, and cobalt. However, other selected components; nitrate ions, phosphate ions, sulfate ions, sodium:potassium ratio, chloride, calcium, magnesium, silicon, manganese, zinc, borate, nickel, molybdenum, tungstate, copper and aluminum, did not enhance 1,3-propanediol production. The use of a fractional factorial design enabled the quick and efficient assessment of the impact of 24 different medium components on 1,3-propanediol production from glycerol from a haloalkaliphilic bacterium. View Full-Text
Keywords: glycerol; 1,3-propanediol; haloalkaliphilic bacteria; fractional factorial design glycerol; 1,3-propanediol; haloalkaliphilic bacteria; fractional factorial design
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Kalia, S.; Trager, J.; Sitton, O.C.; Mormile, M.R. The Use of a Fractional Factorial Design to Determine the Factors That Impact 1,3-Propanediol Production from Glycerol by Halanaerobium hydrogeniformans. Life 2016, 6, 35.

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