Next Article in Journal / Special Issue
Reconciling Ligase Ribozyme Activity with Fatty Acid Vesicle Stability
Previous Article in Journal
Consequences of Decreased Light Harvesting Capability on Photosystem II Function in Synechocystis sp. PCC 6803
Previous Article in Special Issue
Growth and Division in a Dynamic Protocell Model
Article Menu

Export Article

Open AccessReview
Life 2014, 4(4), 915-928; doi:10.3390/life4040915

Toward Spatially Regulated Division of Protocells: Insights into the E. coli Min System from in Vitro Studies

Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Am Klopferspitz 18, Martinsried 82152, Germany
*
Author to whom correspondence should be addressed.
Received: 24 October 2014 / Revised: 25 November 2014 / Accepted: 3 December 2014 / Published: 11 December 2014
(This article belongs to the Special Issue Protocells - Designs for Life)
View Full-Text   |   Download PDF [536 KB, uploaded 11 December 2014]   |  

Abstract

For reconstruction of controlled cell division in a minimal cell model, or protocell, a positioning mechanism that spatially regulates division is indispensable. In Escherichia coli, the Min proteins oscillate from pole to pole to determine the division site by inhibition of the primary divisome protein FtsZ anywhere but in the cell middle. Remarkably, when reconstituted under defined conditions in vitro, the Min proteins self-organize into spatiotemporal patterns in the presence of a lipid membrane and ATP. We review recent progress made in studying the Min system in vitro, particularly focusing on the effects of various physicochemical parameters and boundary conditions on pattern formation. Furthermore, we discuss implications and challenges for utilizing the Min system for division site placement in protocells. View Full-Text
Keywords: protocell; bottom-up synthetic biology; cell division; Min proteins; membranes; self-organization; pattern formation protocell; bottom-up synthetic biology; cell division; Min proteins; membranes; self-organization; pattern formation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Kretschmer, S.; Schwille, P. Toward Spatially Regulated Division of Protocells: Insights into the E. coli Min System from in Vitro Studies. Life 2014, 4, 915-928.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Life EISSN 2075-1729 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top