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Life 2013, 3(1), 181-188; doi:10.3390/life3010181
Communication

Pivotal Enzyme in Glutamate Metabolism of Poly-g-Glutamate-Producing Microbes

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Received: 9 November 2012; in revised form: 28 January 2013 / Accepted: 5 February 2013 / Published: 6 February 2013
(This article belongs to the Special Issue Extremophiles and Extreme Environments)
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Abstract: The extremely halophilic archaeon Natrialba aegyptiaca secretes the L-homo type of poly-g-glutamate (PGA) as an extremolyte. We examined the enzymes involved in glutamate metabolism and verified the presence of L-glutamate dehydrogenases, L-aspartate aminotransferase, and L-glutamate synthase. However, neither glutamate racemase nor D-amino acid aminotransferase activity was detected, suggesting the absence of sources of D-glutamate. In contrast, D-glutamate-rich PGA producers mostly possess such intracellular sources of D-glutamate. The results of our present study indicate that the D-glutamate-anabolic enzyme “glutamate racemase” is pivotal in the biosynthesis of PGA.
Keywords: L-glutamate sources; glutamate racemase; PGA chirality; archaea; bacteria L-glutamate sources; glutamate racemase; PGA chirality; archaea; bacteria
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Ashiuchi, M.; Yamamoto, T.; Kamei, T. Pivotal Enzyme in Glutamate Metabolism of Poly-g-Glutamate-Producing Microbes. Life 2013, 3, 181-188.

AMA Style

Ashiuchi M, Yamamoto T, Kamei T. Pivotal Enzyme in Glutamate Metabolism of Poly-g-Glutamate-Producing Microbes. Life. 2013; 3(1):181-188.

Chicago/Turabian Style

Ashiuchi, Makoto; Yamamoto, Takashi; Kamei, Tohru. 2013. "Pivotal Enzyme in Glutamate Metabolism of Poly-g-Glutamate-Producing Microbes." Life 3, no. 1: 181-188.

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