Growth Suppression of a Robust Bacterium Methylobacterium extorquens by Porous Materials with Oxygen Functional Groups
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Synthesis of Resorcinol–Formaldehyde Gel (RF) and Hydrothermally Treated RF Gel (RFH)
2.3. Characterization of the Porous Materials (RF, RFH, and WS)
2.4. Microbiological Culture Tests of M. extorquens
2.4.1. Preparation of Methanol-Mediated Salts (MMS) Agar Medium
2.4.2. Preparation of Stock Solution of M. extorquens
2.4.3. Culture Tests of M. extorquens with the Porous Materials
3. Results
3.1. Characterization of the Porous Materials
3.1.1. Elemental Analysis
3.1.2. Morphology
3.1.3. Textual Properties
3.1.4. Oxygen Functional Group
3.2. Culture Tests of M. extorquens
3.2.1. Culturing M. extorquens without the Porous Materials (Control Experiment)
3.2.2. Culturing M. extorquens with the Porous Materials
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Element | Percentage in Weight/% | ||
---|---|---|---|
RF | RFH | WS | |
oxygen | 38.3 | 32.4 | 55.2 |
silicone | 0 | 0 | 34.4 |
carbon | 61.6 | 67.6 | 3.2 |
other elements | 0.1 | 0 | 7.2 1 |
Sample ID | SBET/m2 g−1 a | VH2O,0.15/cm3(STP) g−1 b | Γ/nm−2 c |
---|---|---|---|
RF | 240 | 50 | 5.2 |
RFH | 260 | 30 | 3.2 |
WS | 120 | 29 | 6.2 |
Type of Porous Material | Properties of the Oxygen Functional Group a | Colony-Forming Units in the Cultured Solution/CFU mL−1 b |
---|---|---|
RF | phenolic group (Γ = 5.2 nm−2) | <1.0 × 103 |
RFH | phenolic group (Γ = 3.2 nm−2) | 6.8 × 107 |
WS | silanol group (Γ = 6.2 nm−2) | 2.7 × 105 |
Blank | - | 1.6 × 108 |
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Mori, T.; Ogawa, Y.; Endo, I.; Matsushima, K.; Noda, J. Growth Suppression of a Robust Bacterium Methylobacterium extorquens by Porous Materials with Oxygen Functional Groups. Life 2023, 13, 2185. https://doi.org/10.3390/life13112185
Mori T, Ogawa Y, Endo I, Matsushima K, Noda J. Growth Suppression of a Robust Bacterium Methylobacterium extorquens by Porous Materials with Oxygen Functional Groups. Life. 2023; 13(11):2185. https://doi.org/10.3390/life13112185
Chicago/Turabian StyleMori, Takeshi, Yuta Ogawa, Izuki Endo, Keiichiro Matsushima, and Jun Noda. 2023. "Growth Suppression of a Robust Bacterium Methylobacterium extorquens by Porous Materials with Oxygen Functional Groups" Life 13, no. 11: 2185. https://doi.org/10.3390/life13112185