Characterization of Serum Metabolome and Proteome Profiles Identifies SNX5 Specific for Pregnancy Failure in Holstein Heifers
Abstract
:1. Introduction
2. Results
2.1. Metabolic Analysis of Bovine Peripheral Blood during the Peri-Implantation Period
2.2. Comparison of Serum Composition between Pregnant, NP Heifers, and Those with the Estrous Cycle
2.3. Global Proteome Analysis of Serum from Day 20 Pregnant and NP Heifers
2.4. Identification of Specific Proteins in Day-22 Blood Serum Detecting NP Heifers
3. Discussion
4. Materials and Methods
4.1. Collection of Bovine Blood Samples
4.2. Metabolome Analysis
4.3. iTRAQ Analysis
4.4. Western Blot Analysis
4.5. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Kusama, K.; Bai, R.; Matsuno, Y.; Ideta, A.; Sakurai, T.; Nagaoka, K.; Hori, M.; Imakawa, K. Characterization of Serum Metabolome and Proteome Profiles Identifies SNX5 Specific for Pregnancy Failure in Holstein Heifers. Life 2022, 12, 309. https://doi.org/10.3390/life12020309
Kusama K, Bai R, Matsuno Y, Ideta A, Sakurai T, Nagaoka K, Hori M, Imakawa K. Characterization of Serum Metabolome and Proteome Profiles Identifies SNX5 Specific for Pregnancy Failure in Holstein Heifers. Life. 2022; 12(2):309. https://doi.org/10.3390/life12020309
Chicago/Turabian StyleKusama, Kazuya, Rulan Bai, Yuta Matsuno, Atsushi Ideta, Toshihiro Sakurai, Kentaro Nagaoka, Masatoshi Hori, and Kazuhiko Imakawa. 2022. "Characterization of Serum Metabolome and Proteome Profiles Identifies SNX5 Specific for Pregnancy Failure in Holstein Heifers" Life 12, no. 2: 309. https://doi.org/10.3390/life12020309