DnaK3 Is Involved in Biogenesis and/or Maintenance of Thylakoid Membrane Protein Complexes in the Cyanobacterium Synechocystis sp. PCC 6803
Abstract
:1. Introduction
2. Materials and Methods
2.1. Growth Conditions
2.2. SDS-PAGE and Immunoblot Analysis
2.3. Complete Deletion of DnaK3 in Synechocystis Cells Grown under LAHG Conditions
2.4. Cell Number and Chlorophyll Concentration Determination
2.5. Electron Microscopy
2.6. Absorbance and Low Temperature (77K) Fluorescence Spectra
2.7. Oxygen Evolution
2.8. Chlorophyll Fluorescence Induction Curves
2.9. P700 Re-Reduction Kinetics
3. Results
3.1. DnaK3 Synthesis is Light-Induced and Essential in the Dark
3.2. Reducing the DnaK3 Content Affects Cell Growth under Heat Stress Conditions
3.3. The dnaK3KD Strain Has a Reduced Pigment Content
3.4. Reducing the DnaK3 Content Results in an Altered PS I-to-PS II Ratio
3.5. The Photosynthetic Activity is Impaired in the DnaK3 Depleted Strain
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Thurotte, A.; Seidel, T.; Jilly, R.; Kahmann, U.; Schneider, D. DnaK3 Is Involved in Biogenesis and/or Maintenance of Thylakoid Membrane Protein Complexes in the Cyanobacterium Synechocystis sp. PCC 6803. Life 2020, 10, 55. https://doi.org/10.3390/life10050055
Thurotte A, Seidel T, Jilly R, Kahmann U, Schneider D. DnaK3 Is Involved in Biogenesis and/or Maintenance of Thylakoid Membrane Protein Complexes in the Cyanobacterium Synechocystis sp. PCC 6803. Life. 2020; 10(5):55. https://doi.org/10.3390/life10050055
Chicago/Turabian StyleThurotte, Adrien, Tobias Seidel, Ruven Jilly, Uwe Kahmann, and Dirk Schneider. 2020. "DnaK3 Is Involved in Biogenesis and/or Maintenance of Thylakoid Membrane Protein Complexes in the Cyanobacterium Synechocystis sp. PCC 6803" Life 10, no. 5: 55. https://doi.org/10.3390/life10050055