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Antibodies 2016, 5(2), 11; doi:10.3390/antib5020011

Generating Recombinant Antibodies to Membrane Proteins through Phage Display

1
Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607-7060, USA
2
AxioMx Inc., a subsidiary of Abcam Plc, Branford, CT 06405, USA
Current address: Meso Scale Diagnostics LLC, Rockville, MD 20850, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Rui Gong
Received: 25 March 2016 / Revised: 21 April 2016 / Accepted: 21 April 2016 / Published: 2 May 2016
View Full-Text   |   Download PDF [1974 KB, uploaded 2 May 2016]   |  

Abstract

One of the most important classes of proteins in terms of drug targets is cell surface membrane proteins, and yet it is a challenging set of proteins for generating high-quality affinity reagents. In this review, we focus on the use of phage libraries, which display antibody fragments, for generating recombinant antibodies to membrane proteins. Such affinity reagents generally have high specificity and affinity for their targets. They have been used for cell staining, for promoting protein crystallization to solve three-dimensional structures, for diagnostics, and for treating diseases as therapeutics. We cover publications on this topic from the past 10 years, with a focus on the various formats of membrane proteins for affinity selection and the diverse affinity selection strategies used. Lastly, we discuss the challenges faced in this field and provide possible directions for future efforts. View Full-Text
Keywords: affinity selection; fragments of antigen binding (Fabs); G-protein coupled receptors (GPCRs); membrane proteins; nanodiscs; phage-display; recombinant antibodies; single-domain antibodies; transfection; virus-like particles (VLPs) affinity selection; fragments of antigen binding (Fabs); G-protein coupled receptors (GPCRs); membrane proteins; nanodiscs; phage-display; recombinant antibodies; single-domain antibodies; transfection; virus-like particles (VLPs)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Huang, R.; Kiss, M.M.; Batonick, M.; Weiner, M.P.; Kay, B.K. Generating Recombinant Antibodies to Membrane Proteins through Phage Display. Antibodies 2016, 5, 11.

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