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Antibodies 2015, 4(3), 197-224; doi:10.3390/antib4030197

Antibody Conjugates: From Heterogeneous Populations to Defined Reagents

1
Center for Radiopharmaceutical Sciences, Paul Scherrer Institute, 5232 Villigen PSI, Switzerland
2
Department of Chemistry and Applied Biosciences, ETH Zurich, 8093 Zurich, Switzerland
*
Author to whom correspondence should be addressed.
Academic Editor: Dimiter S. Dimitrov
Received: 13 June 2015 / Revised: 2 July 2015 / Accepted: 14 July 2015 / Published: 3 August 2015
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Abstract

Monoclonal antibodies (mAbs) and their derivatives are currently the fastest growing class of therapeutics. Even if naked antibodies have proven their value as successful biopharmaceuticals, they suffer from some limitations. To overcome suboptimal therapeutic efficacy, immunoglobulins are conjugated with toxic payloads to form antibody drug conjugates (ADCs) and with chelating systems bearing therapeutic radioisotopes to form radioimmunoconjugates (RICs). Besides their therapeutic applications, antibody conjugates are also extensively used for many in vitro assays. A broad variety of methods to functionalize antibodies with various payloads are currently available. The decision as to which conjugation method to use strongly depends on the final purpose of the antibody conjugate. Classical conjugation via amino acid residues is still the most common method to produce antibody conjugates and is suitable for most in vitro applications. In recent years, however, it has become evident that antibody conjugates, which are generated via site-specific conjugation techniques, possess distinct advantages with regard to in vivo properties. Here, we give a comprehensive overview on existing and emerging strategies for the production of covalent and non-covalent antibody conjugates. View Full-Text
Keywords: immunoglobulin; bioconjugation; antibody conjugates; chemical conjugation; enzymatic conjugation; non-covalent antibody conjugates immunoglobulin; bioconjugation; antibody conjugates; chemical conjugation; enzymatic conjugation; non-covalent antibody conjugates
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Dennler, P.; Fischer, E.; Schibli, R. Antibody Conjugates: From Heterogeneous Populations to Defined Reagents. Antibodies 2015, 4, 197-224.

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