Next Article in Journal
Opportunities for Conformation-Selective Antibodies in Amyloid-Related Diseases
Previous Article in Journal
Pre-Clinical Intravenous Serum Pharmacokinetics of Albumin Binding and Non-Half-Life Extended Nanobodies®
Article Menu

Export Article

Open AccessArticle
Antibodies 2015, 4(3), 157-169; doi:10.3390/antib4030157

Model-Based Comparison of Antibody Dimerization in Continuous and Batch-Wise Downstream Processing

Department of Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden
*
Author to whom correspondence should be addressed.
Academic Editor: Zivko Nikolov
Received: 31 March 2015 / Revised: 23 June 2015 / Accepted: 6 July 2015 / Published: 10 July 2015
(This article belongs to the Special Issue Optimized Antibody Recovery and Purification Methods)
View Full-Text   |   Download PDF [208 KB, uploaded 10 July 2015]   |  

Abstract

Monoclonal antibodies are generally produced using a generic platform approach in which several chromatographic separations assure high purity of the product. Dimerization can occur during the fermentation stage and may occur also during the downstream processing. We present here simulations in which a traditional platform approach that consist of protein A capture, followed by cation-exchange and anion-exchange chromatography for polishing is compared to a continuous platform in which dimer removal and virus inactivation are carried out on a size-exclusion column. A dimerization model that takes pH, salt concentration and the concentration of antibodies into account is combined with chromatographic models, to be able to predicted both the separation and the degree to which dimers are formed. Purification of a feed composition that contained 1% by weight of dimer and a total antibody concentration of 1 g/L was modeled using both approaches, and the amount of antibodies in the continuous platform was at least 4 times lower than in the traditional platform. The total processing time was also lower, as the cation-exchange polish could be omitted. View Full-Text
Keywords: dimerization; downstream processing; modeling dimerization; downstream processing; modeling
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Sellberg, A.; Ojala, F.; Nilsson, B. Model-Based Comparison of Antibody Dimerization in Continuous and Batch-Wise Downstream Processing. Antibodies 2015, 4, 157-169.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Antibodies EISSN 2073-4468 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top