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Antibodies 2014, 3(2), 232-241; doi:10.3390/antib3020232

Refolding Technologies for Antibody Fragments

1
Alliance Protein Laboratories, 6042 Cornerstone Court West, San Diego, CA 92121, USA
2
Institute for Innovation, Ajinomoto Co. Inc., Kanagawa 210-8681, Japan
*
Author to whom correspondence should be addressed.
Received: 20 January 2014 / Revised: 26 April 2014 / Accepted: 13 May 2014 / Published: 23 May 2014
(This article belongs to the Special Issue Antibody Constructs)
View Full-Text   |   Download PDF [343 KB, 26 May 2014; original version 23 May 2014]   |  

Abstract

Refolding is one of the production technologies for pharmaceutical grade antibody fragments. Detergents and denaturants are primarily used to solubilize the insoluble proteins. The solubilized and denatured proteins are refolded by reducing the concentration of the denaturants or detergents. Several refolding technologies have been used for antibody fragments, comprising dilution, dialysis, solid phase solvent exchange and size exclusion chromatography, as reviewed here. Aggregation suppressor or folding-assisting agents, including arginine hydrochloride, ionic liquids and detergents or denaturants at low concentrations, are included in the refolding solvent to enhance refolding yield.
Keywords: refolding; dialysis; dilution; C12-L-Glu; antibody; fragments refolding; dialysis; dilution; C12-L-Glu; antibody; fragments
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Arakawa, T.; Ejima, D. Refolding Technologies for Antibody Fragments. Antibodies 2014, 3, 232-241.

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