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Antibodies 2013, 2(3), 501-516; doi:10.3390/antib2030501
Article

Development of an Antibody for Detection of Rhamnolipids Characterized as a Major Bacterial Virulence Factor

,  and *
Laboratory of Biochemistry, Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
* Author to whom correspondence should be addressed.
Received: 20 June 2013 / Revised: 4 August 2013 / Accepted: 16 August 2013 / Published: 28 August 2013
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Abstract

Rhamnolipids (RLs), the glycolipidic biosurfactants found initially as exoproducts of the opportunistic pathogen Pseudomonas aeruginosa, are characterized as virulence factors contributing to its pathogenesis infections. However, RLs are also produced by various bacterial species. They consist of a gluconic part, usually containing one or two rhamnoses, and a lipid part, containing one or two hydroxy-fatty acids. In this study, we present both the isolation of RLs from bacterial cultures of the non-pathogenic bacterium Thermus thermophilus as well as the development of the rabbit antibody directed against them. The antibody was titrated and evaluated, in respect of its recognition selectivity. Between both RLs constituents, it specifically recognized only the hydroxydecanoic acid between the fatty acids tested, contrary to rhamnose. The potential of the antibody to recognize both purified RLs and RLs present in crude extracellular media produced by T. thermophilus and Escherichia coli cultures, is evidenced by Dot Blot immuno-reaction. The development of this antibody is addressed in detail, as the sensitive analytical technique, and its potential use would facilitate the implementation of rhamnolipids’ detection, or may be a useful and promising tool for determining these microbial secondary metabolites and virulence factors secreted in extracellular culture media or in biological fluids during infections.
Keywords: virulence; rhamnolipids; antibody; Thermus thermophilus virulence; rhamnolipids; antibody; Thermus thermophilus
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Giagkas, D.C.; Choli-Papadopoulou, T.; Pantazaki, A.A. Development of an Antibody for Detection of Rhamnolipids Characterized as a Major Bacterial Virulence Factor. Antibodies 2013, 2, 501-516.

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