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Genes 2018, 9(8), 413; https://doi.org/10.3390/genes9080413

Proteome-Wide Analysis of Trypanosoma cruzi Exponential and Stationary Growth Phases Reveals a Subcellular Compartment-Specific Regulation

1
Department of Parasitology, Institute of Biomedical Sciences, University of Sao Paulo, 05508-900 Sao Paulo, Brazil
2
Department of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense, Denmark
3
Thermo Fisher Scientific, San Jose, CA 95134, USA
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 8 July 2018 / Revised: 4 August 2018 / Accepted: 4 August 2018 / Published: 15 August 2018
(This article belongs to the Special Issue Membrane Proteins in Parasitic Protozoa)
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Abstract

Trypanosoma cruzi, the etiologic agent of Chagas disease, cycles through different life stages characterized by defined molecular traits associated with the proliferative or differentiation state. In particular, T. cruzi epimastigotes are the replicative forms that colonize the intestine of the Triatomine insect vector before entering the stationary phase that is crucial for differentiation into metacyclic trypomastigotes, which are the infective forms of mammalian hosts. The transition from proliferative exponential phase to quiescent stationary phase represents an important step that recapitulates the early molecular events of metacyclogenesis, opening new possibilities for understanding this process. In this study, we report a quantitative shotgun proteomic analysis of the T. cruzi epimastigote in the exponential and stationary growth phases. More than 3000 proteins were detected and quantified, highlighting the regulation of proteins involved in different subcellular compartments. Ribosomal proteins were upregulated in the exponential phase, supporting the higher replication rate of this growth phase. Autophagy-related proteins were upregulated in the stationary growth phase, indicating the onset of the metacyclogenesis process. Moreover, this study reports the regulation of N-terminally acetylated proteins during growth phase transitioning, adding a new layer of regulation to this process. Taken together, this study reports a proteome-wide rewiring during T. cruzi transit from the replicative exponential phase to the stationary growth phase, which is the preparatory phase for differentiation. View Full-Text
Keywords: Trypanosoma cruzi; metacyclogenesis; exponential and stationary phases; quantitative proteomics; Gim5a; N-acetyltransferases; protein N-terminal acetylation; autophagy Trypanosoma cruzi; metacyclogenesis; exponential and stationary phases; quantitative proteomics; Gim5a; N-acetyltransferases; protein N-terminal acetylation; autophagy
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Avila, C.C.; Mule, S.N.; Rosa-Fernandes, L.; Viner, R.; Barisón, M.J.; Costa-Martins, A.G.; de Oliveira, G.S.; Teixeira, M.M.G.; Marinho, C.R.F.; Silber, A.M.; Palmisano, G. Proteome-Wide Analysis of Trypanosoma cruzi Exponential and Stationary Growth Phases Reveals a Subcellular Compartment-Specific Regulation. Genes 2018, 9, 413.

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