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Genes 2018, 9(1), 56; https://doi.org/10.3390/genes9010056

Identification of a Common Different Gene Expression Signature in Ischemic Cardiomyopathy

1
Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital and Institutes of Biomedical Sciences, Fudan University, 180 Fenglin Road, Shanghai 200032, China
2
Shanghai Institute of clinical bioinformatics, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai 200032, China
3
Institutes of Biomedical Sciences, Fudan University, 130 Dong’an Road, Shanghai 200032, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 20 December 2017 / Revised: 9 January 2018 / Accepted: 16 January 2018 / Published: 22 January 2018
(This article belongs to the Section Human Genomics and Genetic Diseases)
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Abstract

The molecular mechanisms underlying the development of ischemic cardiomyopathy (ICM) remain poorly understood. Gene expression profiling is helpful to discover the molecular changes taking place in ICM. The aim of this study was to identify the genes that are significantly changed during the development of heart failure caused by ICM. The differentially expressed genes (DEGs) were identified from 162 control samples and 227 ICM patients. PANTHER was used to perform gene ontology (GO), and Reactome for pathway enrichment analysis. A protein–protein interaction network was established using STRING and Cytoscape. A further validation was performed by real-time polymerase chain reaction (RT-PCR). A total of 255 common DEGs was found. Gene ontology, pathway enrichment, and protein–protein interaction analysis showed that nucleic acid-binding proteins, enzymes, and transcription factors accounted for a great part of the DEGs, while immune system signaling and cytokine signaling displayed the most significant changes. Furthermore, seven hub genes and nine transcription factors were identified. Interestingly, the top five upregulated DEGs were located on chromosome Y, and four of the top five downregulated DEGs were involved in immune and inflammation signaling. Further, the top DEGs were validated by RT-PCR in human samples. Our study explored the possible molecular mechanisms of heart failure caused by ischemic heart disease. View Full-Text
Keywords: ischemic cardiomyopathy; microarrays; bioinformatical analysis ischemic cardiomyopathy; microarrays; bioinformatical analysis
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Li, Y.; Jiang, Q.; Ding, Z.; Liu, G.; Yu, P.; Jiang, G.; Yu, Z.; Yang, C.; Qian, J.; Jiang, H.; Zou, Y. Identification of a Common Different Gene Expression Signature in Ischemic Cardiomyopathy. Genes 2018, 9, 56.

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