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Genes 2018, 9(1), 50; https://doi.org/10.3390/genes9010050

Single-Cell Genomic Analysis in Plants

1
School of Biological Sciences and Institute of Agriculture, The University of Western Australia, Perth, WA 6009, Australia
2
School of Agriculture and Food Science, The University of Queensland, Brisbane, QLD 4072, Australia
*
Author to whom correspondence should be addressed.
Received: 13 December 2017 / Revised: 5 January 2018 / Accepted: 10 January 2018 / Published: 22 January 2018
(This article belongs to the Special Issue Plant Genomics and Epigenomics for Trait Improvement)
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Abstract

Individual cells in an organism are variable, which strongly impacts cellular processes. Advances in sequencing technologies have enabled single-cell genomic analysis to become widespread, addressing shortcomings of analyses conducted on populations of bulk cells. While the field of single-cell plant genomics is in its infancy, there is great potential to gain insights into cell lineage and functional cell types to help understand complex cellular interactions in plants. In this review, we discuss current approaches for single-cell plant genomic analysis, with a focus on single-cell isolation, DNA amplification, next-generation sequencing, and bioinformatics analysis. We outline the technical challenges of analysing material from a single plant cell, and then examine applications of single-cell genomics and the integration of this approach with genome editing. Finally, we indicate future directions we expect in the rapidly developing field of plant single-cell genomic analysis. View Full-Text
Keywords: computational algorithms; DNA sequencing; plant; single cell analysis; technologies computational algorithms; DNA sequencing; plant; single cell analysis; technologies
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Yuan, Y.; Lee, H.; Hu, H.; Scheben, A.; Edwards, D. Single-Cell Genomic Analysis in Plants. Genes 2018, 9, 50.

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