Methods for Using Small Non-Coding RNAs to Improve Recombinant Protein Expression in Mammalian Cells
AbstractThe ability to produce recombinant proteins by utilizing different “cell factories” revolutionized the biotherapeutic and pharmaceutical industry. Chinese hamster ovary (CHO) cells are the dominant industrial producer, especially for antibodies. Human embryonic kidney cells (HEK), while not being as widely used as CHO cells, are used where CHO cells are unable to meet the needs for expression, such as growth factors. Therefore, improving recombinant protein expression from mammalian cells is a priority, and continuing effort is being devoted to this topic. Non-coding RNAs are RNA segments that are not translated into a protein and often have a regulatory role. Since their discovery, major progress has been made towards understanding their functions. Non-coding RNA has been investigated extensively in relation to disease, especially cancer, and recently they have also been used as a method for engineering cells to improve their protein expression capability. In this review, we provide information about methods used to identify non-coding RNAs with the potential of improving recombinant protein expression in mammalian cell lines. View Full-Text
Share & Cite This Article
Inwood, S.; Betenbaugh, M.J.; Shiloach, J. Methods for Using Small Non-Coding RNAs to Improve Recombinant Protein Expression in Mammalian Cells. Genes 2018, 9, 25.
Inwood S, Betenbaugh MJ, Shiloach J. Methods for Using Small Non-Coding RNAs to Improve Recombinant Protein Expression in Mammalian Cells. Genes. 2018; 9(1):25.Chicago/Turabian Style
Inwood, Sarah; Betenbaugh, Michael J.; Shiloach, Joseph. 2018. "Methods for Using Small Non-Coding RNAs to Improve Recombinant Protein Expression in Mammalian Cells." Genes 9, no. 1: 25.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.