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Genes 2017, 8(7), 171; doi:10.3390/genes8070171

The Role of Replication-Associated Repair Factors on R-Loops

Andalusian Center for Molecular Biology and Regenerative Medicine-CABIMER, Universidad de Sevilla-CSIC-Universidad Pablo de Olavide, Av. Américo Vespucio 24, 41092 Seville, Spain
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Academic Editor: Frédéric Chedin
Received: 4 May 2017 / Revised: 19 June 2017 / Accepted: 20 June 2017 / Published: 27 June 2017
(This article belongs to the Special Issue R-loop Biology in Eukaryotes)
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Abstract

The nascent RNA can reinvade the DNA double helix to form a structure termed the R-loop, where a single-stranded DNA (ssDNA) is accompanied by a DNA-RNA hybrid. Unresolved R-loops can impede transcription and replication processes and lead to genomic instability by a mechanism still not fully understood. In this sense, a connection between R-loops and certain chromatin markers has been reported that might play a key role in R-loop homeostasis and genome instability. To counteract the potential harmful effect of R-loops, different conserved messenger ribonucleoprotein (mRNP) biogenesis and nuclear export factors prevent R-loop formation, while ubiquitously-expressed specific ribonucleases and DNA-RNA helicases resolve DNA-RNA hybrids. However, the molecular events associated with R-loop sensing and processing are not yet known. Given that R-loops hinder replication progression, it is plausible that some DNA replication-associated factors contribute to dissolve R-loops or prevent R-loop mediated genome instability. In support of this, R-loops accumulate in cells depleted of the BRCA1, BRCA2 or the Fanconi anemia (FA) DNA repair factors, indicating that they play an active role in R-loop dissolution. In light of these results, we review our current view of the role of replication-associated DNA repair pathways in preventing the harmful consequences of R-loops. View Full-Text
Keywords: genetic instability; DNA-RNA hybrids; Fanconi anemia; BRCA; replication stress; cancer genetic instability; DNA-RNA hybrids; Fanconi anemia; BRCA; replication stress; cancer
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Bhatia, V.; Herrera-Moyano, E.; Aguilera, A.; Gómez-González, B. The Role of Replication-Associated Repair Factors on R-Loops. Genes 2017, 8, 171.

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