Role of Archaeal HerA Protein in the Biology of the Bacterium Thermus thermophilus
AbstractIntense gene flux between prokaryotes result in high percentage of archaeal genes in the genome of the thermophilic bacteria Thermus spp. Among these archaeal genes a homolog to the Sulfolobus spp. HerA protein appears in all of the Thermus spp. strains so far sequenced (HepA). The role of HepA in Thermus thermophilus HB27 has been analyzed using deletion mutants, and its structure resolved at low resolution by electron microscopy. Recombinant HepA shows DNA-dependent ATPase activity and its structure revealed a double ring, conically-shaped hexamer with an upper diameter of 150 Å and a bottom module of 95 Å. A central pore was detected in the structure that ranges from 13 Å at one extreme, to 30 Å at the other. Mutants lacking HepA show defective natural competence and DNA donation capability in a conjugation-like process termed “transjugation”, and also high sensitivity to UV and dramatic sensitivity to high temperatures. These data support that acquisition of an ancestral archaeal HerA has been fundamental for the adaptation of Thermus spp. to high temperatures. View Full-Text
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Blesa, A.; Quintans, N.G.; Baquedano, I.; Mata, C.P.; Castón, J.R.; Berenguer, J. Role of Archaeal HerA Protein in the Biology of the Bacterium Thermus thermophilus. Genes 2017, 8, 130.
Blesa A, Quintans NG, Baquedano I, Mata CP, Castón JR, Berenguer J. Role of Archaeal HerA Protein in the Biology of the Bacterium Thermus thermophilus. Genes. 2017; 8(5):130.Chicago/Turabian Style
Blesa, Alba; Quintans, Nieves G.; Baquedano, Ignacio; Mata, Carlos P.; Castón, José R.; Berenguer, José. 2017. "Role of Archaeal HerA Protein in the Biology of the Bacterium Thermus thermophilus." Genes 8, no. 5: 130.
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