Next Article in Journal
NAD1 Controls Defense-Like Responses in Medicago truncatula Symbiotic Nitrogen Fixing Nodules Following Rhizobial Colonization in a BacA-Independent Manner
Next Article in Special Issue
Transcriptional-Readthrough RNAs Reflect the Phenomenon of “A Gene Contains Gene(s)” or “Gene(s) within a Gene” in the Human Genome, and Thus Are Not Chimeric RNAs
Previous Article in Journal
High-Throughput Sequencing of Small RNA Transcriptomes in Maize Kernel Identifies miRNAs Involved in Embryo and Endosperm Development
Previous Article in Special Issue
Connections between Transcription Downstream of Genes and cis-SAGe Chimeric RNA
Article Menu
Issue 12 (December) cover image

Export Article

Open AccessArticle
Genes 2017, 8(12), 386; doi:10.3390/genes8120386

Absence of Correlation between Chimeric RNA and Aging

1
Department of Pathology, School of Medicine, University of Virginia, Charlottesville, VA 22908, USA
2
National Institute of Plant Genome Research (NIPGR), New Delhi 110067, India
3
Department of Biochemistry and Molecular Genetics, School of Medicine, University of Virginia, Charlottesville, VA 22908, USA
*
Author to whom correspondence should be addressed.
Received: 18 November 2017 / Revised: 6 December 2017 / Accepted: 7 December 2017 / Published: 14 December 2017
View Full-Text   |   Download PDF [726 KB, uploaded 19 December 2017]   |  

Abstract

Chimeric RNAs have been recognized as a phenomenon not unique to cancer cells. They also exist in normal physiology. Aging is often characterized by deregulation of molecular and cellular mechanisms, including loss of heterochromatin, increased transcriptional noise, less tight control on alternative splicing, and more stress-induced changes. It is thus assumed that chimeric RNAs are more abundant in older people. In this study, we conducted a preliminary investigation to identify any chimeric RNAs with age-based trends in their expression levels in blood samples. A chimeric RNA candidate list generated by bioinformatic analysis indicated the possibility of both negative and positive trends in the expression of chimeric RNAs. Out of this candidate list, five novel chimeric RNAs were successfully amplified in multiple blood samples and then sequenced. Although primary smaller sample sizes displayed some weak trends with respect to age, analysis of quantitative PCR data from larger sample sizes showed essentially no relationship between expression levels and age. Altogether, these results indicate that, contradictory to the common assumption, chimeric RNAs as a group are not all higher in older individuals and that placing chimeric RNAs in the context of aging will be a much more complex task than initially anticipated. View Full-Text
Keywords: chimeric RNA; aging; cis-splicing of adjacent genes; trans-splicing chimeric RNA; aging; cis-splicing of adjacent genes; trans-splicing
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Share & Cite This Article

MDPI and ACS Style

Huang, R.; Kumar, S.; Li, H. Absence of Correlation between Chimeric RNA and Aging. Genes 2017, 8, 386.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top