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Genes 2017, 8(12), 380; doi:10.3390/genes8120380

The Alteration of Nasopharyngeal and Oropharyngeal Microbiota in Children with MPP and Non-MPP

1
Department of Respiratory Diseases, Shenzhen Children’s Hospital, Shenzhen 518026, China
2
Department of Computer Science, City University of Hong Kong, Hong Kong 999077, China
3
Department of Microbial Research, WeHealthGene Institute, Shenzhen 518129, China
4
Institute of Statistics, NanKai University, Tianjin 300071, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 12 October 2017 / Revised: 5 December 2017 / Accepted: 5 December 2017 / Published: 11 December 2017
(This article belongs to the Section Microbial Genetics and Genomics)
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Abstract

Background: In recent years, the morbidity of Mycoplasma pneumoniae pneumonia (MPP) has increased significantly in China. A growing number of studies indicate that imbalanced respiratory microbiota is associated with various respiratory diseases. Methods: We enrolled 119 children, including 60 pneumonia patients and 59 healthy children. Nasopharyngeal (NP) and oropharyngeal (OP) sampling was performed for 16S ribosomal RNA (16S rRNA) gene analysis of all children. Sputum and OP swabs were obtained from patients for pathogen detection. Results: Both the NP and OP microbiota of patients differ significantly from that of healthy children. Diseased children harbor lower microbial diversity and a simpler co-occurrence network in NP and OP. In pneumonia patients, NP and OP microbiota showed greater similarities between each other, suggesting transmission of NP microbiota to the OP. Aside from clinically detected pathogens, NP and OP microbiota analysis has also identified possible pathogens in seven cases with unknown infections. Conclusion: NP and OP microbiota in MPP and non-MPP are definitely similar. Respiratory infection generates imbalanced NP microbiota, which has the potential to transmit to OP. Microbiota analysis also promises to compliment the present means of detecting respiratory pathogens. View Full-Text
Keywords: 16S rDNA; microbiota; Mycoplasma pneumoniae; nasopharyngeal; oropharyngeal 16S rDNA; microbiota; Mycoplasma pneumoniae; nasopharyngeal; oropharyngeal
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MDPI and ACS Style

Lu, Z.; Dai, W.; Liu, Y.; Zhou, Q.; Wang, H.; Li, D.; Yang, Z.; Li, Y.; Xie, G.; Li, S.; Zheng, Y. The Alteration of Nasopharyngeal and Oropharyngeal Microbiota in Children with MPP and Non-MPP. Genes 2017, 8, 380.

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