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Genes 2017, 8(12), 345; doi:10.3390/genes8120345

Characterization of the Transcriptome and Gene Expression of Tetraploid Black Locust Cuttings in Response to Etiolation

1
Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
2
State Key Laboratory of Forest Genetics and Tree Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
3
The State-Owned Luoning County Lv Cun Forest Farm, Luoning 471711, China
4
The State-Owned Ji County Nursery of Forests, Linfen 042200, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 21 September 2017 / Revised: 2 November 2017 / Accepted: 11 November 2017 / Published: 24 November 2017
(This article belongs to the Special Issue Novel Insights into the Genetics of Root Development)
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Abstract

Etiolation (a process of growing plants in partial or complete absence of light) promotes adventitious root formation in tetraploid black locust (Robinia pseudoacacia L.) cuttings. We investigated the mechanism underlying how etiolation treatment promotes adventitious root formation in tetraploid black locust and assessed global transcriptional changes after etiolation treatment. Solexa paired-end sequencing of complementary DNAs (cDNAs) from control (non-etiolated, NE) and etiolated (E) samples resulted in 107,564 unigenes. In total, 52,590 transcripts were annotated and 474 transcripts (211 upregulated and 263 downregulated) potentially involved in etiolation were differentially regulated. These genes were associated with hormone metabolism and response, photosynthesis, signaling pathways, and starch and sucrose metabolism. In addition, we also found significant differences of phytohormone contents, activity of following enzymes i.e., peroxidase, polyphenol oxidase and indole acetic acid oxidase between NE and E tissues during some cottage periods. The genes responsive to etiolation stimulus identified in this study will provide the base for further understanding how etiolation triggers adventitious roots formation in tetraploid black locus. View Full-Text
Keywords: Tetraploid Robinia pseudoacacia L.; etiolation; transcriptome; phytohormone; peroxidase activity Tetraploid Robinia pseudoacacia L.; etiolation; transcriptome; phytohormone; peroxidase activity
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MDPI and ACS Style

Lu, N.; Dai, L.; Luo, Z.; Wang, S.; Wen, Y.; Duan, H.; Hou, R.; Sun, Y.; Li, Y. Characterization of the Transcriptome and Gene Expression of Tetraploid Black Locust Cuttings in Response to Etiolation. Genes 2017, 8, 345.

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