Next Article in Journal
Integrative miRNA-Gene Expression Analysis Enables Refinement of Associated Biology and Prediction of Response to Cetuximab in Head and Neck Squamous Cell Cancer
Next Article in Special Issue
RNA Editing, ADAR1, and the Innate Immune Response
Previous Article in Journal
Replication Fork Protection Factors Controlling R-Loop Bypass and Suppression
Previous Article in Special Issue
Identification and Analysis of RNA Editing Sites in the Chloroplast Transcripts of Aegilops tauschii L.
Article Menu

Export Article

Open AccessArticle
Genes 2017, 8(1), 34; doi:10.3390/genes8010034

Applying Human ADAR1p110 and ADAR1p150 for Site-Directed RNA Editing—G/C Substitution Stabilizes GuideRNAs against Editing

Interfaculty Institute of Biochemistry, University of Tübingen, Auf der Morgenstelle 15, 72076 Tübingen, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: H. Ulrich Göringer
Received: 25 November 2016 / Revised: 23 December 2016 / Accepted: 6 January 2017 / Published: 14 January 2017
(This article belongs to the Special Issue RNA Editing)
View Full-Text   |   Download PDF [2109 KB, uploaded 14 January 2017]   |  

Abstract

Site-directed RNA editing is an approach to reprogram genetic information at the RNA level. We recently introduced a novel guideRNA that allows for the recruitment of human ADAR2 to manipulate genetic information. Here, we show that the current guideRNA design is already able to recruit another human deaminase, ADAR1, in both isoforms, p110 and p150. However, further optimization seems necessary as the current design is less efficient for ADAR1 isoforms. Furthermore, we describe hotspots at which the guideRNA itself is edited and show a way to circumvent this auto-editing without losing editing efficiency at the target. Both findings are important for the advancement of site-directed RNA editing as a tool in basic biology or as a platform for therapeutic editing. View Full-Text
Keywords: site-directed RNA editing; ADAR; guideRNA; genetic disease; RNA repair site-directed RNA editing; ADAR; guideRNA; genetic disease; RNA repair
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Heep, M.; Mach, P.; Reautschnig, P.; Wettengel, J.; Stafforst, T. Applying Human ADAR1p110 and ADAR1p150 for Site-Directed RNA Editing—G/C Substitution Stabilizes GuideRNAs against Editing. Genes 2017, 8, 34.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top