Next Article in Journal
Replication Fork Protection Factors Controlling R-Loop Bypass and Suppression
Previous Article in Journal
Acknowledgement to Reviewers of Genes in 2016
Article Menu

Export Article

Open AccessArticle
Genes 2017, 8(1), 31; doi:10.3390/genes8010031

Characterization of the Transcriptome and Gene Expression of Brain Tissue in Sevenband Grouper (Hyporthodus septemfasciatus) in Response to NNV Infection

Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University, Yeosu 550-749, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: J. Peter W. Young
Received: 10 November 2016 / Revised: 7 January 2017 / Accepted: 9 January 2017 / Published: 13 January 2017
(This article belongs to the Section Microbial Genetics and Genomics)
View Full-Text   |   Download PDF [1442 KB, uploaded 13 January 2017]   |  

Abstract

Grouper is one of the favorite sea food resources in Southeast Asia. However, the outbreaks of the viral nervous necrosis (VNN) disease due to nervous necrosis virus (NNV) infection have caused mass mortality of grouper larvae. Many aqua-farms have suffered substantial financial loss due to the occurrence of VNN. To better understand the infection mechanism of NNV, we performed the transcriptome analysis of sevenband grouper brain tissue, the main target of NNV infection. After artificial NNV challenge, transcriptome of brain tissues of sevenband grouper was subjected to next generation sequencing (NGS) using an Illumina Hi-seq 2500 system. Both mRNAs from pooled samples of mock and NNV-infected sevenband grouper brains were sequenced. Clean reads of mock and NNV-infected samples were de novo assembled and obtained 104,348 unigenes. In addition, 628 differentially expressed genes (DEGs) in response to NNV infection were identified. This result could provide critical information not only for the identification of genes involved in NNV infection, but for the understanding of the response of sevenband groupers to NNV infection. View Full-Text
Keywords: nervous necrosis virus (NNV); sevenband grouper; transcriptome; next generation sequencing (NGS); differential expressed genes (DEGs) nervous necrosis virus (NNV); sevenband grouper; transcriptome; next generation sequencing (NGS); differential expressed genes (DEGs)
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Kim, J.-O.; Kim, J.-O.; Kim, W.-S.; Oh, M.-J. Characterization of the Transcriptome and Gene Expression of Brain Tissue in Sevenband Grouper (Hyporthodus septemfasciatus) in Response to NNV Infection. Genes 2017, 8, 31.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top