Next Article in Journal
Segregation of Incomplete Achromatopsia and Alopecia Due to PDE6H and LPAR6 Variants in a Consanguineous Family from Pakistan
Next Article in Special Issue
The Balance between Recombination Enzymes and Accessory Replicative Helicases in Facilitating Genome Duplication
Previous Article in Journal
Transcriptomic Analysis of the Endangered Neritid Species Clithon retropictus: De Novo Assembly, Functional Annotation, and Marker Discovery
Previous Article in Special Issue
Stationary-Phase Mutagenesis in Stressed Bacillus subtilis Cells Operates by Mfd-Dependent Mutagenic Pathways
Article Menu

Export Article

Open AccessReview
Genes 2016, 7(8), 40; doi:10.3390/genes7080040

Replication Termination: Containing Fork Fusion-Mediated Pathologies in Escherichia coli

Division of Biosciences, College of Health and Life Sciences, Brunel University London, Uxbridge UB8 3PH, UK
*
Author to whom correspondence should be addressed.
Academic Editor: Richard T. Pomerantz
Received: 27 May 2016 / Revised: 12 July 2016 / Accepted: 19 July 2016 / Published: 25 July 2016
(This article belongs to the Special Issue Replication and Transcription Associated DNA Repair)
View Full-Text   |   Download PDF [5165 KB, uploaded 25 July 2016]   |  

Abstract

Duplication of bacterial chromosomes is initiated via the assembly of two replication forks at a single defined origin. Forks proceed bi-directionally until they fuse in a specialised termination area opposite the origin. This area is flanked by polar replication fork pause sites that allow forks to enter but not to leave. The precise function of this replication fork trap has remained enigmatic, as no obvious phenotypes have been associated with its inactivation. However, the fork trap becomes a serious problem to cells if the second fork is stalled at an impediment, as replication cannot be completed, suggesting that a significant evolutionary advantage for maintaining this chromosomal arrangement must exist. Recently, we demonstrated that head-on fusion of replication forks can trigger over-replication of the chromosome. This over-replication is normally prevented by a number of proteins including RecG helicase and 3’ exonucleases. However, even in the absence of these proteins it can be safely contained within the replication fork trap, highlighting that multiple systems might be involved in coordinating replication fork fusions. Here, we discuss whether considering the problems associated with head-on replication fork fusion events helps us to better understand the important role of the replication fork trap in cellular metabolism. View Full-Text
Keywords: termination of DNA replication; fork collisions; RecG; homologous recombination; co-orientation of replication and transcription termination of DNA replication; fork collisions; RecG; homologous recombination; co-orientation of replication and transcription
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Dimude, J.U.; Midgley-Smith, S.L.; Stein, M.; Rudolph, C.J. Replication Termination: Containing Fork Fusion-Mediated Pathologies in Escherichia coli. Genes 2016, 7, 40.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top