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Genes 2016, 7(12), 127; doi:10.3390/genes7120127

New Cross-Talk Layer between Ultraconserved Non-Coding RNAs, MicroRNAs and Polycomb Protein YY1 in Bladder Cancer

1
Institute of Genetics and Biophysics—CNR. Via P. Castellino, 111, 80131 Naples, Italy
2
Bioker srl multimedica spa, via Brin, 49/65 80142 Naples, Italy
3
Dipartimento di Farmacia, Università di Napoli “Federico II”, 80131 Naples, Italy
4
Department of Translational Medical Sciences, University of Naples “Federico II”, 80131 Naples, Italy
5
Division of Urology, European Institute of Oncology, 20141 Milan, Italy
6
Division of Urology, IRCS National Tumor Institute, 80131 Naples, Italy
7
Institute of Protein Biochemistry—CNR. Via P. Castellino, 111, 80131 Naples, Italy
8
Department of Biochemistry, Biophysic and General Pathology, University of Campania Luigi Vanvitelli, Via De Crecchio 7, 80138 Naples, Italy
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editors: George A. Calin and Muller Fabbri
Received: 5 October 2016 / Revised: 23 November 2016 / Accepted: 1 December 2016 / Published: 14 December 2016
(This article belongs to the Special Issue microRNAs and Other Non-Coding RNAs in Human Diseases)
View Full-Text   |   Download PDF [1150 KB, uploaded 14 December 2016]   |  

Abstract

MicroRNAs (miRNAs) are highly conserved elements in mammals, and exert key regulatory functions. Growing evidence shows that miRNAs can interact with another class of non-coding RNAs, so-called transcribed ultraconserved regions (T-UCRs), which take part in transcriptional, post-transcriptional and epigenetic regulation processes. We report here the interaction of miRNAs and T-UCRs as a network modulating the availability of these non-coding RNAs in bladder cancer cells. In our cell system, antagomiR-596 increased the expression of T-UCR 201+. Moreover, T-UCR 8+ silencing increased miR-596 expression, which in turn reduced total T-UCR 283+, showing that the perturbation of one element in this network changes the expression of other interactors. In addition, we identify the polycomb protein Yin Yang 1 (YY1) as mediator of binding between miR-596 and T-UCR 8+. These new findings describe for the first time a network between T-UCRs, miRNAs and YY1 protein, highlighting the existence of an additional layer of gene expression regulation. View Full-Text
Keywords: transcribed ultraconserved regions; microRNAs; bladder cancer; interaction network transcribed ultraconserved regions; microRNAs; bladder cancer; interaction network
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Terreri, S.; Durso, M.; Colonna, V.; Romanelli, A.; Terracciano, D.; Ferro, M.; Perdonà, S.; Castaldo, L.; Febbraio, F.; de Nigris, F.; Cimmino, A. New Cross-Talk Layer between Ultraconserved Non-Coding RNAs, MicroRNAs and Polycomb Protein YY1 in Bladder Cancer. Genes 2016, 7, 127.

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