• Abstract
• Full-Text PDF [691 KB]
• Full-Text XML
• Article Versions Notes
• Supplement File 1
• Supplement File 2
• Supplement File 3
• Supplement File 4

• Article Statistics
• Google Scholar
• Order Reprints

• Cite this article
• Export to BibTeX
• Export to EndNote

• [+] on DOAJ
• Wasukira, A
• Tayebwa, J
• Thwaites, R
• Paszkiewicz, K
• Aritua, V
• Kubiriba, J
• Smith, J
• Grant, M
• Studholme, D
• [+] on Google Scholar
• Wasukira, A
• Tayebwa, J
• Thwaites, R
• Paszkiewicz, K
• Aritua, V
• Kubiriba, J
• Smith, J
• Grant, M
• Studholme, D
• [+] on PubMed
• Wasukira, A
• Tayebwa, J
• Thwaites, R
• Paszkiewicz, K
• Aritua, V
• Kubiriba, J
• Smith, J
• Grant, M
• Studholme, D

•  CiteULike
•  Facebook
•  Mendeley
•  Twitter

This article is

• freely available
• re-usable

Genes 2012, 3(3), 361-377; doi:10.3390/genes3030361

Communication

Genome-Wide Sequencing Reveals Two Major Sub-Lineages in the Genetically Monomorphic Pathogen Xanthomonas Campestris Pathovar Musacearum

Arthur Wasukira ^1,2 , Johnbosco Tayebwa ^1,2 , Richard Thwaites ³ , Konrad Paszkiewicz ¹ , Valente Aritua ^2,3,† , Jerome Kubiriba ² , Julian Smith ³ , Murray Grant ¹  and David J. Studholme ^1,*

¹ Biosciences, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter EX4 4QD, UK ² National Crops Resources Research Institute (NaCRRI), Kampala 7084, Uganda ³ The Food and Environment Research Agency, Sand Hutton, York YO41 1LZ, UK ^† Present address: International Centre for Tropical Agriculture (CIAT), Kawanda Agricultural Research Institute, Kampala 6247, Uganda.

* Author to whom correspondence should be addressed.

Received: 10 June 2012; in revised form: 24 June 2012 / Accepted: 26 June 2012 / Published: 4 July 2012

(This article belongs to the Special Issue Feature Paper 2012)

Download PDF Full-Text [691 KB, uploaded 4 July 2012 08:31 CEST]

Abstract: The bacterium Xanthomonas campestris pathovar musacearum (Xcm) is the causal agent of banana Xanthomonas wilt (BXW). This disease has devastated economies based on banana and plantain crops (Musa species) in East Africa. Here we use genome-wide sequencing to discover a set of single-nucleotide polymorphisms (SNPs) among East African isolates of Xcm. These SNPs have potential as molecular markers for phylogeographic studies of the epidemiology and spread of the pathogen. Our analysis reveals two major sub-lineages of the pathogen, suggesting that the current outbreaks of BXW on Musa species in the region may have more than one introductory event, perhaps from Ethiopia. Also, based on comparisons of genome-wide sequence data from multiple isolates of Xcm and multiple strains of X. vasicola pathovar vasculorum, we identify genes specific to Xcm that could be used to specifically detect Xcm by PCR-based methods.

Keywords: banana Xanthomonas wilt; whole-genome sequencing; SNP; molecular markers; Ensete ventricosum; Xanthomonas vasicola

Supplementary Files

• Supplementary File 1:

  PDF-Document (PDF, 376 KB)

• Supplementary File 2:

  PDF-Document (PDF, 198 KB)

• Supplementary File 3:

  PDF-Document (PDF, 132 KB)

• Supplementary File 4:

  PDF-Document (PDF, 357 KB)

Article Statistics

Cite This Article