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Cells 2018, 7(4), 28; https://doi.org/10.3390/cells7040028

Flotillins Regulate Focal Adhesions by Interacting with α-Actinin and by Influencing the Activation of Focal Adhesion Kinase

1
Institute of Biochemistry, Medical Faculty, University of Giessen, Friedrichstrasse 24, 35392 Giessen, Germany
2
Department of Medicine 2, Hematology/Oncology, University Hospital, Goethe University Frankfurt, 60590 Frankfurt am Main, Germany
3
Image Computing & Information Technologies, Kapersburgstrasse 12, 60437 Frankfurt, Germany
4
Center for Membrane Proteomics, University of Frankfurt, Max-von-Laue Strasse 13, 60438 Frankfurt am Main, Germany
*
Author to whom correspondence should be addressed.
Received: 26 February 2018 / Revised: 4 April 2018 / Accepted: 6 April 2018 / Published: 7 April 2018
(This article belongs to the Special Issue Cell Adhesion Molecules)
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Abstract

Cell–matrix adhesion and cell migration are physiologically important processes that also play a major role in cancer spreading. In cultured cells, matrix adhesion depends on integrin-containing contacts such as focal adhesions. Flotillin-1 and flotillin-2 are frequently overexpressed in cancers and are associated with poor survival. Our previous studies have revealed a role for flotillin-2 in cell–matrix adhesion and in the regulation of the actin cytoskeleton. We here show that flotillins are important for cell migration in a wound healing assay and influence the morphology and dynamics of focal adhesions. Furthermore, anchorage-independent growth in soft agar is enhanced by flotillins. In the absence of flotillins, especially flotillin-2, phosphorylation of focal adhesion kinase and extracellularly regulated kinase is diminished. Flotillins interact with α-actinin, a major regulator of focal adhesion dynamics. These findings are important for understanding the molecular mechanisms of how flotillin overexpression in cancers may affect cell migration and, especially, enhance metastasis formation. View Full-Text
Keywords: focal adhesion; cancer; metastasis; cell migration focal adhesion; cancer; metastasis; cell migration
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Banning, A.; Babuke, T.; Kurrle, N.; Meister, M.; Ruonala, M.O.; Tikkanen, R. Flotillins Regulate Focal Adhesions by Interacting with α-Actinin and by Influencing the Activation of Focal Adhesion Kinase. Cells 2018, 7, 28.

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